| 低氧大鼠肺动脉内皮细胞VEGF变化与PKC活性关系的探讨 |
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| 引用本文： | 杨曦明,谢印芝,聂鸿靖,尹昭云.低氧大鼠肺动脉内皮细胞VEGF变化与PKC活性关系的探讨[J].中国应用生理学杂志,2000,16(3):250-253. |
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| 作者姓名： | 杨曦明 谢印芝 聂鸿靖 尹昭云 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津,300050 |
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| 基金项目： | 国家自然科学基金!资助项目 (39730 190 ) |
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| 摘 要： | 目的：探讨低氧培养大鼠肺动脉血管内皮细胞ＶＥＧＦ的表达变化与ＰＫＣ活性的关系。方法：培养大鼠肺动脉血管内皮细胞，观察低氧（１％Ｏ２）培养不同时间大鼠肺动脉血管内皮细胞浆、膜ＰＫＣ活性和培养液中ＶＥＧＦ水平变化；加入ＰＫＣ抑制剂（ｓｔａｕｒｏｓｐｏｒｉｎｅ）后，测定低氧、常氧培养不同时间二者的变化。结果：低氧时膜ＰＫＣ活性和培养液中ＶＥＧＦ水平明显升高（Ｐ＜０．０１）。而加入ＰＫＣ抑制剂后，常氧和低
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| 关 键 词： | 低氧 肺动脉血管内皮细胞 蛋白激酶 |
| 修稿时间： | 2000-01-17 |
THE RELATIONSHIP BETWEEN VEGF LEVEL AND PKC ACTIVITY IN PULMONARY ARTERIAL VASCULAR ENDOTHELIAL CELLS OF RAT DURING HYPOXIA |
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| YANG Xi-ming,XIE Yin-zhi,NIE Hong-jing,YIN Zhao-yun.THE RELATIONSHIP BETWEEN VEGF LEVEL AND PKC ACTIVITY IN PULMONARY ARTERIAL VASCULAR ENDOTHELIAL CELLS OF RAT DURING HYPOXIA[J].Chinese Journal of Applied Physiology,2000,16(3):250-253. |
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| Authors: | YANG Xi-ming XIE Yin-zhi NIE Hong-jing YIN Zhao-yun |
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| Abstract: | Aim:To study the relationship between VEGF expression and PKC activity in pulmonary arterial vascular endothelial cells during hypoxia. Methods:We detected the changes of PKC activity and VEGF levels in pulmonary arterial vascular endothelial cells under the hypoxia,hypoxia with PKC inhibitor and normoxia with PKC inhibitor conditions. Results:It was showed that both PKC activity and VEGF levels increased significantly ( P <0.01) during hypoxia. After adding PKC inhibitor (staurosporine), however,they were both inhibited under the hypoxia and normoxia conditions. Conclusion:Hypoxia stimulates pulmonary arterial vascular endothelial cells to secrete VEGF and PKC may be one of the factors that up-regulate VEGF gene expression during hypoxia. |
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| Keywords: | hypoxia pulmonary arterial vascular endothelial cells VEGF PKC |
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