| 丹参素对肝肺综合征大鼠肺脏的保护作用 |
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| 引用本文： | 贾建桃,张慧英,来丽娜,李旭炯,田小霞,张丽丽,吕敏丽,赵中夫,韩德五,Cheng ji.丹参素对肝肺综合征大鼠肺脏的保护作用[J].中国应用生理学杂志,2014(3):199-203,I0001. |
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| 作者姓名： | 贾建桃 张慧英 来丽娜 李旭炯 田小霞 张丽丽 吕敏丽 赵中夫 韩德五 Cheng ji |
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| 作者单位： | [1]长治医学院病理生理学教研室,山西长治046000 [2]长治医学院药理学教研室,山西长治046000 [3]长治医学院生理学教研室,山西长治046000 [4]山西医科大学第二医院ICU,太原030001 [5]长治医学院肝病研究所,山西长治046000 [6]山西医科大学肝病研究所,太原030001 [7]美国南加州大学KECK医学院肝病研究中心,加利福尼亚州洛杉矶90089 |
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| 基金项目： | 国家自然科学基金资助项目（81070339）;山西省国际科技合作项目（2010081068）;山西医科大学细胞生理学省部共建教育部重点实验室主任基金（2010-09）;山西省高等学校科技创新项目资助（2010120）;山西省回国留学人员资助项目（211-091） |
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| 摘 要： | 目的：探讨丹参素减轻肝肺综合征大鼠肺组织炎性损伤的作用。方法：SD大鼠被随机分为正常对照组（n=8）、肝肺综合征组（n=11）和丹参素干预组（n=9）。采用HE染色观察肝及肺组织病理改变，计数肺组织巨噬细胞；测定血浆丙氨酸转氨酶（ALT）的活性以及内毒素、肿瘤坏死因子α（TNF-α）和同型半胱氨酸（Hcy）的浓度及肺组织匀浆中TNF-α、一氧化氮（NO）和丙二醛（MDA）的含量和诱导型一氧化氮合酶（iNOS）的活性。结果：与正常对照组相比，肝肺综合征组动物肺泡腔变小、间隔增厚、肺泡腔内和间隔内有大量巨噬细胞聚集，且体积明显增大。与肝肺综合征组相比，丹参素干预组肺组织巨噬细胞数量明显减少、病理改变显著减轻。肝肺综合征组大鼠血浆ALT的活性和内毒素、TNF-α、Hcy的浓度以及肺组织中TNF-α、NO和MDA含量以及iNOS的活性，均高于正常对照组，而使用丹参素干预后各指标均明显降低。结论：丹参素可能通过降低肠源性内毒素，减轻肺组织的炎性反应，延缓肝肺综合征的进展。
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| 关 键 词： | 肝肺综合征 肠源性内毒素血症 丹参素 巨噬细胞 |
Protective effect of tanshinol on the hepatopulmonary syndrome in rat |
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| Institution: | JIA Jian-tao, ZHANG Hui-ying, LAI Li-na, LI Xu-jiong, TIAN Xiao-xia, ZHANG Li-li, LV Min-li, ZHAO Zhong-fu, HAN De-wu, CHENG Ji(1. Department of Pathophysiology, 2. Department of Pharmacology, 3. Department of Physiology, Changzhi Medical College, Changzhi 046000; 4. ICU of the Second Hospital, Shanxi Medical University, Taiyuan 030001; 5. Liver Disease Institute, Changzhi Medical College, Changzhi 046000; 6. Liver Disease Institute, Shanxi Medical University, Taiyuan 030001, China; 7. USC Research Center for Liver Disease, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA) |
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| Abstract: | Objective： To explore the mechanism of tanshinol on alleviate the inflammatory injury of lung tissue in rat hepatopulmonary syndrome （HPS）. Melhods： SD rats were randomly divided into normal control group（ n = 8）, hepatopulmonary syndrome （HPS） group（ n = 11 ） and tanshinol intervention group（ n = 9）. HE staining was used to observe the histopathology changes of pulmonary and hepatic tissues, and to count the number of macrophages in lung tissues. The activity of alanine transferase （ALT） and concentrations of endotoxin, tumor necrosis factor-α （TNF-α） and homocystein （Hcy） in plasma were detected. The concentrations of TNF-α, nitric oxide （NO） and malondialdehyde （MDA） and the activity of inducible nitric oxide synthase （iNOS） in the lung tissues were measured, respectively. Results： Thickened alveolar septum and increased macrophages were observed in lungs in HPS rat. After administered with tanshinol, the pulmonary pathological changes were alleviated and the number of macrophages in lung tissue was decreased compared with HPS group. The activity of ALT and the concentrations of endotoxin, TNF-α and Hey in plasma ,and TNF-α, iNOS, NO and MDA in lung tissue in HPS group were higher than those of normal control group; meanwhile, those tanshinol group were less those that of HPS group. Conclusion： Tanshinol may play an important role in delaying the development of lIPS through protecting liver or directly antagonizing the effect of intestinal endotoxemia so as to alleviate the inflammatory reaction in lung tissue. |
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| Keywords: | hepatopulmonary syndrome intestinal endotoxemia tanshinol macrophage |
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