血必净注射液对缺氧／复氧大鼠心肌TLR4-NF-KB-IL-1β通路的影响

目的：探讨血必净注射液（XBJI）对缺氧／复氧大鼠心肌TLR4-NF-KB-IL-1β通路的抑制作用。方法：健康雄性sD大鼠36只，体重（280±30）g，随机分为6组（n=6）：正常组（N组）、平衡灌注组（BP组）、模型组（M组）、小剂量xBⅡ组（xBJII组）、中剂量XBJI组（XBJIM组）、大剂量XBJI组（XBJIH组）。通过langendorff离体心脏灌流装置，建立缺氧／复氧大鼠心肌炎性反应模型。用ELISA方法检测心肌组织中白介素113（IL-1β）的浓度；Westernblot检测心肌组织中核因子一xt3p65（NF-xB065）蛋白、T0Ⅱ样受体4（1184）蛋白的表达；RT-PCR检测心肌组织中NF-td3p65mR—NA及TLR4mRNA的表达；光镜观察其心肌光学显微结构的改变。结果：与M组比较，XBJII．组、XBJIM组、XBJIn组心肌IL-113的浓度、NF-KB-065及TLR4蛋白、NF_出p65及TLR4mRNA表达量有不同程度的下降，均以XBJIM组下降最为明显（P〈0．05，P〈0．01）；M组心肌结构损伤严重，XBJI处理后上述变化均得到改善，以XBJIM最为明显。结论：不同剂量XBJI可通过抑制TLR4-NF-KB-IL-1β信号转导通路减轻缺氧／复氧大鼠心肌的炎性反应，以4ml／100rnl的XBJI疗效最佳。

Effect of Xuebijing injection on TLR4--NF-KB--IL-1β pathway of myocardial hypoxia/reoxygenation in rats

Objective： To investigate the role of Xuebijing injection（XBJI, traditional Chinese medicine）, in inhibiting TLR4--NF IL-1/3 pathway of myocardial hypoxia/reoxygenation in rats. Methods： Thirty six male SD rats （280 _＋ 30）g were randomly divided into six groups（ n = 6） ： normal group （ N group）, balanced peffusion group （ BP group）, model group （ M group）, low dose XBJI group（ XBJIL group）, middle dose XBJI group（XBJIM group）, high dose XBJI group （XBJIH group）. By langendorff isolated heart perfusion device to establish the model of myocardial hypoxia/reoxygenation in rats. ELISA was used to detect the concentration of interleukin-1β （IL-1β）; Western blot was used to detect the expression of nuclear factor kappa B p65 （ NF-xB 1365 ）protein and toll like receptor 4（TLR4 ） protein; and RT-PCR to deter- mine the expression of NF-xB p65 mRNA and TLR4 mRNA;To observe microstrueture changes of hypoxia/reoxygenation myocardial by light microscopy. Results： Compared with M group,the IL-l~3 eoneentration,NF-tcB 1365 and TLR4 protein,NF-xB p65 and TLR4 mRNA of XBJIL group,XBJ/M group, XBJIH group expression decreased in varying degrees,and decreased most obviously all in XBJIM group（ P 〈 0.05, P 〈 0. 01 ）; Myocardical structural damage was serious in M group,and improved after treatment XBJI,the most obvious was the XBJIM. Conclusion： Different dose of XBJI can inhibit TLR4--NF-xB--IL- 1β signal transduction pathway and reduce several inflammatory reaction after myocardial hypoxia/reoxygenation iniury,the 4 ml/100 rnl of XBJI is the best.