高湿环境对大鼠免疫功能的影响

目的：观察高湿环境下大鼠免疫功能的变化，并探讨其意义，为进一步研究相关影响机制奠定基础。方法：将30只sD大鼠随机分为3组（n：10）：分别为常温常湿组、湿化20d组和40d组。湿化模型组用人工环境气候箱模拟高湿环境，在90％的湿度条件下进行处理，并于第20天、40天分别处死，收集外周血和脾脏，用流式细胞术检测大鼠外周血和脾脏的T淋巴细胞的百分率。结果：湿化20d大鼠外周血CD3’％为52．91±6．27，CD4％为37．80±4．11，CD8＋％为14．85±3．73，CD4＋／CD8’为2．72±0．82，除CD3％外与常温常湿组均有显著性差异（P〈0．05），而40d组外周血T淋巴细胞亚群数据均无统计学差异。湿化各组脾脏T淋巴细胞的百分率与常温常湿组差异不大，20d组CD8’％为6．23±2．87，有显著性差异（P〈0．05）。血清检测结果显示，高湿组IgG、IgA、IgM均未见显著变化，而20d组c3显著升高。结论：在高湿环境下，初期大鼠的免疫自稳状态发生变化，免疫功能升高，随着时间的推移，机体通过自我的调整，可能逐渐习服，免疫功能逐渐降低到正常水平。

Effect of high humidity environment on immune function in rats

Objective： To investigate effects of the variation of immune function in high humidity environment in different time, and lay a foundation for further study of the related mechanism. Method： Thirty SD rots were divided into 3 groups （ n = 10）： 20 day group,40 day group in 90% relative humidity chamber and control group in normal relative humidity. Peripheral blood and spleens were collected to detect the levels of T lymphocyte subsets by Flow Cytometery. Results： In peripheral blood of the 20 day group rats, the CD3＋ %, CD4＋ %, CD8 ~ % and CD4 ~/CD8 ~ were 52.91 ＋ 6.27,37.80 ＋ 4.11,14.85 ~： 3.73 and 2.72 ＋ 0.82 separately. Expect CD3 ~ %, they all had sig- nificant differences （ P 〈 0.05）. In addition, the data of the 40 day group rats showed no diversity in statistics. In spleen, CD8 ~ % of the 20 day group rots was 6.23 ＋ 2.87 with significant differences （ P 〈 0.05） and IgG, IgA and IgM did not change a lot in blood serum of the high humidity groups except C3 of the 20 days group （ P 〈 0.05）. Conclusion： In high humidity environment, the immune function of the rats in- creased in the initial stage. As time went on, the immune function gradually went to normal level through the self adjustment.