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育阴软肝颗粒剂对肝纤维化大鼠TGF-β1表达的影响
引用本文:王志旺,付晓艳,程小丽,包小英,刘雪枫,段海婧.育阴软肝颗粒剂对肝纤维化大鼠TGF-β1表达的影响[J].中国应用生理学杂志,2018,34(2):169-172.
作者姓名:王志旺  付晓艳  程小丽  包小英  刘雪枫  段海婧
作者单位:1. 甘肃中医药大学, 兰州 730000;2. 甘肃中医药大学附属医院, 兰州 730000
基金项目:甘肃省高等学校科研基金项目(2015A-096)
摘    要:目的:观测育阴软肝颗粒剂对大鼠肝纤维化模型的防治作用及对转化生长因子-β1(TGF-β1)表达的影响。方法:将Wistar大鼠分为6组(n=10),注射四氯化碳、饲以高脂饲料并饮用20%乙醇6周复制肝纤维化大鼠模型,经6.2~24.8 g/kg育阴软肝颗粒剂干预(qd)6周后,测定肝纤维化大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性、透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(C-Ⅳ)及板层素(LN)含量,观测肝组织病理学及肝组织TGF-β1表达的变化,对育阴软肝颗粒剂防治肝纤维作用及机制进行研究。结果:实验第7周,模型组大鼠肝组织出现明显的纤维化病变(P<0.01);与模型组比较,6.2~24.8g/kg的育阴软肝颗粒剂能明显降低肝指数以及血清ALT、AST活性与HA、PCⅢ、C-Ⅳ、LN含量,缓解肝组织纤维化病理变化,抑制纤维化肝组织TGF-β1的表达(P<0.05,0.01)。结论:育阴软肝颗粒剂对多因素复制肝纤维化大鼠造模具有明显的治疗作用,而抑制TGF-β1的表达可能是其作用机制之一。

关 键 词:肝纤维化  大鼠  四氯化碳  育阴软肝颗粒剂  β1-转化生长因子  
收稿时间:2017-04-13

Effect of Yuyin Ruangan Granule on TGF-β1 expression in hepatic fibrosis rats
WANG Zhi-wang,FU Xiao-yan,CHENG Xiao-li,BAO Xiao-ying,LIU Xue-feng,DUAN Hai-jing.Effect of Yuyin Ruangan Granule on TGF-β1 expression in hepatic fibrosis rats[J].Chinese Journal of Applied Physiology,2018,34(2):169-172.
Authors:WANG Zhi-wang  FU Xiao-yan  CHENG Xiao-li  BAO Xiao-ying  LIU Xue-feng  DUAN Hai-jing
Institution:1. Gansu University of Chinese Medicine, Lanzhou 730000;2. Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China
Abstract:Objective:To observe the preventive and therapeutic action of Yuyin Ruangan Granule (YRG, Traditional Chinese Medicine) in hepatic fibrosis rats model and its effect on transforming growth factor-β1 (TGF-β1) expression. Methods:The Wistar rats were randomly divided into 6 group (n=10), and the model of hepatic fibrosis rats was established by subcutaneous injected with carbon tetrachloride (CCL4), fed on high-fat diet and 20% ethanol for 6 weeks, to survey the effect and mechanism of YRG preventing hypatic fibrosis by detecting liver function (the activity of alanine aminotransferase(ALT), aspartate aminotransferase(AST), etc.) of liver fibrosis rats, liver fibrosis indicators (hyaluronic acid, Ⅲ procollagen, type IV collagen, laminin and hepatic pathology, etc.), and TGF-β1 expression in liver tissue after 6 weeks treated with YRG through intragastric administration (q. d.). Results:At the 7th week, fibrotic lesions appears distinctly in liver tissue of model group compared with control group (P<0.01), YRG of 6.2~28.8 g/kg could significantly decrease hepatic index, ALT and AST activities, content of hyaluronic acid(HA), Ⅲ procollagen (PCⅢ), type Ⅳ collagen(C-Ⅳ), laminin (LN) in serum, relieve liver fibrosis pathological changes and inhibit TGF-β1 expression in fibrotic liver tissue (P<0.05, P<0.01). Conclusion:YRG has significantly preventive effects on liver fibrosis rats model, and it may be one of its mechanisms to inhibit expression of TGF-β1.
Keywords:hepatic fibrosis  rat  carbon tetrachloride  Yuyin Ruangan Granule (YRG)  β1-transformating growth factor (TGF-β1)  
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