红芪总多糖及红芪多糖HG-1对大鼠骨关节炎的影响

目的：探讨红芪总多糖（HPS）及红芪多糖HG-1（HHG-1）对大鼠骨关节炎的治疗作用及机制。方法：将Wistar大鼠随机分为空白对照组、模型对照组、阳性对照组（透明质酸钠1.00 mg/joint）、HPS高低剂量组（HPS 1.05、0.35 mg/joint）、HHG-1高低剂量组（HHG-1 0.45、0.15 mg/joint）共7组（n=8），采用木瓜蛋白酶局部注射复制骨关节炎动物模型，经HPS及HHG-1的干预，测定关节活动范围、滑膜病理及其中糖胺多糖，全血粘度及血清抗氧化系统等指标变化，对HPS及HHG-1治疗骨关节炎的作用及机制进行研究。结果：HPS （0.35、1.05 mg/joint）及HHG-1（0.15、0.45 mg/joint）能明显增大骨关节炎大鼠膝关节的伸展角度，缓解关节软骨病理变化，抑制关节面滑膜异常增厚，提高滑膜中糖胺多糖的含量；同时HPS及HHG-1可降低大鼠血液粘度，提高血清超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性，降低丙二醛（MDA）的含量（P<0.05，P<0.01）。结论：HPS及HHG-1对骨关节炎有一定的治疗作用，但二者的疗效无明显差异；改善血液流变学而缓解软骨代谢异常、提高抗氧化酶活性而缓解软骨损伤是其作用机制之一。

Effects of Hedysari Polysaccharides and Hedysari Polysaccharide HG-1 on osteoarthritis in rats

Objective: To investigate the therapeutic effects and mechanisms of Hedysari Polysaccharides (HPS) and Hedysari Polysaccharide HG-1 (HHG-1) on osteoarthritis (OA) of rats.Methods: Wistar rats were randomly divided into seven groups with eight in each as following:normal control group, model control group, positive control group(Sodium hyaluronate, SHA 1.00 mg/joint), HPS treated group (HPS 0.35 or 1.05 mg/join), and HHG-1 treated group(HHG-1 0.15 or 0.45 mg/joint). The rat's OA model was established with papain. The range of joint activity, synovial pathology and its glycosaminoglycan (GAG) content, whole blood viscosity, and serum antioxidant indexes were determined after treated with HPS and HHG-1.Results: HPS (0.35, 1.05 mg/joint) and HHG-1 (0.15, 0.45 mg/joint) could significantly enlarge the stretching angle of knee joint, relieve the pathological changes of articular cartilage, inhibit abnormal thickening of articular synovium, increase the content of GAG in synovi in the OA' rats. Meanwhile, HPS and HHG-1 could reduce the blood viscosity, enhance the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), decrease the serum level of malondialdehyde (MDA) (P<0.05, P<0.01).Conclusion: HPS and HHG-1 have some therapeutic effects on OA, but there is no significant difference between the two groups. Its possible mechanisms are improving hemorheology to alleviate abnormal cartilage metabolism and improving the activity of antioxidant enzymes to relieve cartilage injury.