内毒诱导肺动脉内皮细胞生成过氧亚硝基阴离子的意义

目的：探讨牛肺动脉内皮细胞（BPAEC）产生过氧亚硝基阴离子（ONOO^-）的能力及其作用。方法：用流式细胞免疫荧光技术,定量检测内毒素主要成分脂多糖（LPS）诱导培养的BPAEC中ONOO^-生成标志物硝基酪氨酸（NT）的含量,观察ONOO^-对BPAEC形态变化的影响。结果：LPS可剂量依赖性诱导BPAEC产生ONOO^-明显增多,并为氮基胍部分翻转;ONOO^-可导致BPAEC明显回缩,胞体变小,细胞间隙增宽。结论：LPS诱导BPAEC产生增多的ONOO^-可能参与介导LPS对BPAEC本身的损伤效应。

Endotoxin-induced generation of peroxynitrite in bovine pulmonary arterial endothelial cells

Aim: To explore the significance of bovine pulmonary arterial endothelial cell (BPAEC) to produce peroxynitrite (ONOO -). Methods: The contents of nitrotyrosine (NT),a marker of ONOO - generation in vivo ,were quantitatively measured with flow cytometric and immunofluorescent technique in cultured BPAEC induced by lipopolysaccharide(LPS),a major component of bacterial endotoxin. In addition,effect of ONOO - on micromorphologic features of BPAEC was observed. Results: LPS caused significant increase of NT in BPAEC in a dose dependent manner,which was in part reversed by aminoguanidine,a selective inhibitor of inducible nitric oxide synthase. Exposure of BPAEC to exogenous ONOO - resulted in the cellular retro contraction with diminshed cellular body and increased width of cellular junction. Conclusion: LPS may induce the ONOO - formation of BPAEC. Endothelial cell derived ONOO - may mediate the deleterious effect of LPS on BPAEC per se .