| 肌肽对低氧所致大鼠血管内皮细胞损伤的保护作用 |
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| 引用本文： | 白剑,迟戈,张俊,刘丽娜,樊淼,刘海东,李伟伟,杨菁.肌肽对低氧所致大鼠血管内皮细胞损伤的保护作用[J].中国应用生理学杂志,2010(1):30-32. |
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| 作者姓名： | 白剑 迟戈 张俊 刘丽娜 樊淼 刘海东 李伟伟 杨菁 |
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| 作者单位： | 辽宁医学院生物化学与分子生物学教研室;辽宁中医药大学;辽宁省食品药品监督管理局技术审评中心;大连医科大学解剖学教研室; |
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| 基金项目： | 辽宁省自然科学基金资助课题(20072202) |
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| 摘 要： | 目的：研究肌肽对低氧所致大鼠血管内皮细胞损伤的影响。方法：建立低氧条件下大鼠血管内皮细胞损伤模型,用MTT法观察肌肽对低氧损伤的血管内皮细胞活性的影响,测定细胞培养基中LDH活力,并对细胞骨架进行考马斯亮蓝R-250染色观测其细胞结构。结果：浓度为10mmol/L～20mmol/L肌肽孵育血管内皮细胞6h后,可以抑制缺氧12h和24h引起的血管内皮细胞活性下降,同时减少LDH的释放,保持细胞骨架完整。结论：肌肽对低氧所致的血管内皮细胞损伤具有保护作用。
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| 关 键 词： | 肌肽 细胞骨架 血管内皮细胞损伤 缺氧 |
Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia |
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| BAI Jian,CHI Ge,ZHANG Jun,LIU Li-na,FAN Miao,LIU Hai-dong,LI Wei-wei,YANG Jing.Protective effect of carnosine on the injury of rat vascular endothelial cells induced by hypoxia[J].Chinese Journal of Applied Physiology,2010(1):30-32. |
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| Authors: | BAI Jian CHI Ge ZHANG Jun LIU Li-na FAN Miao LIU Hai-dong LI Wei-wei YANG Jing |
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| Institution: | BAI Jian1,CHI Ge2,ZHANG Jun3,LIU Li-na1,FAN Miao1,LIU Hai-dong1,LI Wei-wei1,YANG Jing1(1.Department of Biochemistry , Molecular Biology,Liaoning Medical University,Jinzhou 110021,China,2.Liaoning Food , Drug Administration Technical Evaluation Center,Shenyang 110001,3.Department of Anatomy,Dalian Medical University,Dalian 116044,China) |
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| Abstract: | Objective:To investigate the effect of camosine on the injury of rat vascular endothelial cells(VECs) induced by hypoxia.Methods:The model of the injury of rat VECs induced by hypoxia was established.The effect of camosine on injury of VECs activity induced by hypoxia was determined by MTT assay.The levels of lactate dehydrogenase (LDH) activity in cell medium were measured with correspon-ding kit.The cell structure was observed under microscope after Coomassie brilliant blue R-250 staining.Results:After cu... |
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| Keywords: | camosine cell structure vascular endothelial cells injury hypoxia |
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