外源性睾酮对去睾丸家兔性激素以及血脂和载脂蛋白水平的影响

目的 :探讨不同剂量的外源性睾酮对去势 (双侧睾丸切除 )雄性家兔性激素以及血脂和载脂蛋白水平的影响。方法 :成熟雄性新西兰白兔 40只 ,随机分成 5组 (n =8) :对照组、单纯去势组、低睾酮血症组 去势后肌注十一酸睾酮 (TU) ,3mg/kg ,每 2周一次 ]、生理水平组 (去势后肌注TU ,6mg/kg ,每 2周一次 )及高睾酮血症组 (去势后肌注TU ,1 2mg/kg ,每 2周一次 )。 8周后测量血清总睾酮 (TT)、雌二醇 (E2 )、脱氢表雄酮 (DHEA)及总胆固醇 (TC)、高密度脂蛋白胆固醇 (HDL C) ,低密度脂蛋白胆固醇 (LDL C) ,甘油三酯 (TG) ,载脂蛋白A1 (ApoA1 ) ,载脂蛋白B(ApoB)。结果 :雄兔血清TT水平在去势后明显下降至极低水平 ,明显低于对照组 (P <0 .0 1 ) ,3mg/kgTU补充后轻度升高 ,仍明显低于对照组 (P <0 .0 1 ) ,形成低睾酮血症 ,6mg/kgTU补充后与对照组相比无显著差异 (P >0 .0 5) ,接近生理水平 ,1 2mg/kgTU补充后明显高于对照组和低睾酮血症组 (P均 <0 .0 1 ) ,形成高睾酮血症。血清E2 水平在对照组和生理水平组相近并且最低 ,单纯去势组和低睾血症组相近 ,高睾酮血症组最高。E2 /TT比值在对照组和生理水平组相近并且最小 ,在单纯去势组最大 ,低睾酮血症组大于高睾酮血症组。单纯去势组、低睾酮血症组和高睾酮血症

Effects of serum testosterone level on plasma lipid and apolipoprotein spectrum in orchiectomized rabbits

Aim: To investigate the effects of serum testosterone level on plasms lipid and apolipoprotein spectrum in orchiectomized rabbits. Methods: 40 male New Zealand rabbits were divided randomly into five groups( n =8): control group, sham(castration), castration group(castration), hypotestosteronemia group(castration plus low dose testosterone replacement), physiological level group(castration plus moderate testosterone replacement), and hypertestosteronemia group(castration plus large dose testosterone replacment). The rabbits in hypotestosteronemia, physiological level and hypertestosteronemia group were injected testosterone undecanoate 3 mg/kg, 6 mg/kg, and 12 mg/kg, respectively and the remaining control, and castration rabbits, vehicle only. 8 weeks after initiation of the experiment, blood was collected for determination of serum levels of total testosterone(TT), estradiol(E 2), lipids, lipoproteins, apolipoproteins. Results: After castration, the serum level of TT decreased significantly to the lowest level, and TU replacement increased serum TT level depending on dose. The low dose of TU increased serum TT level slightly and produced a hypotestosteronemia model, the moderate dose of TU increased serum TT level near to normal level and produced a physiological model, the large dose of TU increased serum TT level significantly to the highest level and produced a hypertestosteronemia model. The tatol cholesterol(TC), triglycerides(TG), low density lipoprotein cholesterol(LDL C), apolipoprotein B(ApoB) were significantly increased in hypotestosteronemia and hypertestosteronemia rabbits and significantly decreased in physiological serum testosterone rabbits. Conclusion: Testosterone, at physiological level has good effect on the serum lipids, lipoproteins, apolipoproteins in the castration rabbits, and the hypotestosteronemia and hypertestosteronemia has bad effect.