复方中药提取物对大鼠不同状态下脑组织自由基代谢影响的研究

目的:探讨复方中药提取物对大鼠脑组织自由基代谢和抗氧化系统能力的影响机制。方法:选取70只健康Wistar大鼠,随机分为2组(n=35):对照组(N)和服药组(M)。适应性喂养1周,服药组大鼠连续服用8周的复方中药提取物,9周后将2组大鼠分别于安静状态、定量负荷、力竭运动即刻、力竭恢复12 h、力竭恢复24 h状态下处死。分别测定上述2组大鼠在不同功能状态下脑组织中丙二醛(MDA)含量,谷胱甘肽过氧化物酶(GSH-PX)、还原性谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)活性。结果:五种状态下,服药组MDA含量均显著低于对照组,GSH-PX、GSH、SOD、T-AOC活性均不同程度的高于对照组。结论:复方中药提取物可降低不同功能状态下大鼠脑组织中的MDA含量,提高其脑组织GSH-PX、GSH、SOD、T-AOC活性。

Effects of the compound extract of Chinese medicine on free radical metabolism of the rat brain in different states

Objective: To investigate the effect of the" compound extract of Chinese medicine" on free radical metabolism and antioxidant enzyme systems of the rat brain.Methods: 70 Wistar mice were randomly divided into two groups(n=35):normal control group(N),taking medicine group(M).After a week of feeding,M group was taking medicine for 8 weeks.After 9 weeks,killed the two groups when they were at the rest state,the immediate ends of the fixed load,the immediate ends of exhaustive exercise and 12,24 hour ends of exhaustive exercise,respectively.And the activity of malonaldehyde(MDA),glutathione peroxidase(GSH-PX),glutathione(GSH),superoxide dismutase(SOD)、total anti oxidation capacity(TAOC) of the rat brain were measured.Results: At the five sates, the content of MDA in M group was lower than that in N group in different degree,the activity of GSH-PX、GSH、SOD、T-AOC in M group were higher than those in N group in different degree.Conclusion: The "Compound extract of Chinese medicine" can reduce the MDA level of the rat brain and improve the enzyme activity of GSH-PX,GSH,SOD,T-AOC.