降钙素基因相关肽对人支气管上皮细胞E-钙粘素表达的影响

目的:探讨降钙素基因相关肽(CGRP)对臭氧(O3)应激后人支气管上皮细胞(HBECs)中E-钙粘素(E-cd)表达的影响及机制。方法:采用RT-PCR检测E-cd mRNA的表达,免疫细胞化学染色法检测E-cd蛋白的表达。结果:CGRP呈剂量依赖性增加正常以及O3应激后HBECs胞膜上E-cd的表达,而对胞浆内E-cd的表达无明显影响;CGRP对HBECs胞膜上E-cd表达的上调作用可分别被H-89(PKA抑制剂)、H-7(PKC抑制剂)及W-7(CaM抑制剂)部分逆转。结论:CGRP可剂量依赖性增加正常和O3应激后HBECs胞膜E-cd的表达,而对胞浆内E-cd的表达无影响。

Effects of CGRP on the E-cadherin expression in human bronchial epithelial cells

Objective: To discuss the effect of calcitonin gene-related peptides(CGRP) on epithelial cadherin(E-cd) expression in human bronchial epithelial cells(HBECs) in vitro.Methods: The effect of CGRP on E-cd protein and mRNA expression in both normal and O3-challenged HBECs were determined by immunocytochemistry and RT-PCR.The signal transduction pathways of CGRP were observed by using protein kinase C(PKC) inhibitor(H-7),calmodulin(CaM) inhibitor(W-7) and PKA inhibitor(H-89).Results: CGRP increased E-cd mRNA and protein expressions of normal and O3-challenged HBECs in a dose-dependent manner.CGRP had no effect on cytoplasm E-cd expression.Pre-treatment with H-89,H-7 and W-7,the up-regulatory effect of CGRP on E-cd expression was partly abolished.Conclusion: CGRP increased in cytomembrane E-cd expression of normal and O3-challenged HBECs in a dose-dependent manner.E-cd expression on HBECs was strengthened by CGRP via PKA,PKC and CaM pathways.