| pEGFP-N1质粒转染乳鼠心肌细胞的分布及效率 |
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| 作者姓名： | Feng H Xiu WL Zhan R Qian LJ |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津,300050 |
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| 摘 要： | 目的: 研究pEGFP-N1质粒转染心肌细胞的分布及效率.方法: 培养乳鼠心肌细胞,根据乳鼠心肌细胞的不同生长时间(1～3 d)进行pEGFP-N1质粒转染心肌细胞的实验研究.结果: 乳鼠心肌细胞生长1 d时,pEGFP-N1质粒转染心肌细胞的效率显著高于乳鼠心肌细胞生长2 d、3 d时;pEGFP-N1质粒转染心肌细胞后EGFP均匀地充满胞浆和胞核.结论: pEGFP-N1质粒转染乳鼠心肌细胞的效率与心肌细胞的生长期有关;EGFP在心肌细胞中均匀分布于胞浆和胞核.
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| 关 键 词： | pEGFP-N1质粒 转染 心肌细胞 分布 转染效率 |
| 文章编号： | 1000-6834(2005)01-0112-03 |
Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes |
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| Feng H,Xiu WL,Zhan R,Qian LJ.Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes[J].Chinese Journal of Applied Physiology,2005,21(1):112-114,i004. |
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| Authors: | Feng Hong Xiu Wen-lin Zhan Rui Qian Ling-jia |
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| Institution: | Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China. |
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| Abstract: | Aim: To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes. Methods: The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied. Results: The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus. Conclusion: The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus. |
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| Keywords: | pEGFP-N1 plasmid transfection cardiomyocyte distribution transfection efficiency |
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