酒精对丙酸睾酮诱导的小鼠前列腺增生的作用

目的：探讨酒精对丙酸睾酮引起的小鼠前列腺增生（BPH）的作用及其生殖毒性。方法：成年雄性昆明系小鼠70只随机分为空白对照组（Control）、阴性对照组（Negative control，sc大豆油25 mg/（kg·d），ig蒸馏水7.5 ml/（kg·d），连续处理7 d、21 d）、酒精7 d和21 d组（AL7和AL21，ig 50°白酒7.5 ml/（kg·d），连续处理7 d、21 d），丙酸睾酮7 d和21 d组（TP7和TP21，sc丙酸睾酮注射液25 mg/（kg·d），连续处理7 d、21 d），丙酸睾酮+酒精7 d组（TP+AL7，sc丙酸睾酮注射液25 mg/（kg·d），ig50°白酒7.5 ml/（kg·d），连续处理7 d），每组10只。末次处理24 h后处死小鼠，计算小鼠前列腺和睾丸系数，检测精子参数，测定睾丸和前列腺组织中自由基水平、抗氧化能力，观察前列腺组织病理学变化。结果：与对照组、TP7 d组、AL7和AL21 d组相比，TP+AL7 d组的前列腺系数显著提高、精子数量和质量显著降低、前列腺和睾丸MDA含量显著升高、SOD和GPx酶活力显著下降（P均< 0.05）；与TP21 d组相比，TP+AL7 d组的前列腺系数无显著差别（P>0.05））。结论：丙酸睾酮和酒精共同处理7 d就可以达到典型的前列腺增生（BPH）状态，并引起睾丸及精子的损伤，导致生殖系统的氧化应激反应增强，说明酒精对丙酸睾酮引起的小鼠前列腺增生有明显的促进作用。

Effects of alcohol on benign prostate hyperplasia induced by testosterone propionate in mice

Objective: To study the effects of alcohol administration on benign prostate hyperplasia(BPH) and the reproductive toxicity during development of benign prostate hyperplasia. Methods: Seventy adult male Kunming mice were randomly divided into seven groups:control (group CON), negative control (group NC, injected subcutaneously with soybean oil, 25 mg/(kg·d), intragastric administration of distilled water, 7.5 ml/(kg·d)), alcohol for 7 and 21 days (group AL7 and AL21, intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)), testosterone propionate for 7 and 21 days (group TP7 and TP21, injected subcutaneously with testosterone propionate, 25 mg/(kg·d)), testosterone propionate+alcohol for 7 days (group TP+AL7, injected subcutaneously with testosterone propionate, 25 mg/(kg·d), and intragastric administration with wine of 50% alcohol, 7.5 ml/(kg·d)),10 mice in each groups. Twenty-four hours after the last administration, mice were sacrificed. The indexes of prostate and testis and the parameters of sperm were determined in mice. The levels of free radicals, antioxidation and histopathological changes in testis and prostate were determined. Results: Compared with the control, TP7d group, AL7 and AL21d groups, the prostate coefficient of TP + AL7d group was increased significantly and the quantity and quality of sperm were decreased significantly (P<0.05), the content of MDA in prostate and testis was increased significantly, meanwhile the activities of SOD and GPx were decreased significantly (P< 0.05). Compared with TP21d group, the prostate coefficient of TP + AL7d group had no significant difference (P>0.05). Conclusion: The typical BPH state could be induced after 7-day treatment of testosterone propionate and alcohol. The testicular and sperm were damaged which enhanced the oxidative stress in reproductive system. The results indicated that alcohol could significantly promote the prostate hyperplasia induced by testosterone propionate in mice.