不同季节高原鼢鼠乳酸脱氢酶活力和同工酶谱

目的:探讨高原鼢鼠对洞道低氧高二氧化碳环境的代谢适应机制。方法:用酶活力分析法,分析春季、夏季和秋季高原鼢鼠血清乳酸脱氢酶(LDH)活力、乳酸含量和组织LDH活力,用聚丙烯酰胺凝胶电泳法分析血清和组织LDH同工酶谱。结果:高原鼢鼠血清LDH活力在春夏秋三季具有明显的差异,春季高于夏季,夏季高于秋季,血清乳酸含量表现出同样的变化趋势;春季血清中五种同工酶条带都清晰可见,夏季血清中LDH5和LDH4清晰可见,秋季血清中只能看见LDH5带。骨骼肌、心肌和脑组织LDH活力较高,而且从春季到秋季显著降低;肝、肾和肺组织LDH活力较低,肝组织LDH活力春季显著高于夏季和秋季,夏秋两季之间没有明显差异;肾和肺组织LDH活力在春季与夏季之间没有明显差异,但秋季明显降低。心、肝、肺、肾、脑和肌肉组织LDH同工酶谱,在春夏秋三季都显示出五条带,并表现出明显的组织差异;各组织同工酶含量也有不同程度的季节差异。结论:高原鼢鼠体内糖酵解过程具有明显的季节性变化,从春季到秋季依次降低,这与它们的季节性活动特点和洞道中氧气和二氧化碳的季节性波动有关。

Activity and isoenzyme spectra of lactate dehydrogenase of plateau zokor (Myospalax baileyi) in different season

Aim:To explore the adapting metabolic mechanisms of the plateau zokors to the hypoxic-hypercapnic environment.Methods:The activities of lactate dehydrogenase in serum and tissues,and the content of lactate in serum of plateau zokors in spring,summer and autumn were determined by using method of enzyme analysis.The spectrums of lactate dehydrogenase isoenzymes in serum and tissues of plateau zokors in spring,summer and autumn were analyzed by using method of the discontinuous systemic poly-acrylamide perpendicular plank gel electrophoresis.Results:The activities of lactate dehydrogenase in serum had obvious seasonally difference that were higher in spring and lower in autumn,and the content of lactate in serum showed same changing pattern.The spectrums of lactate dehydrogenase isoenzymes in serum showed five bands that were LDH1,LDH2,LDH3,LDH4 and LDH5 from positive pole to negative pole respectively,it showed clearly two bands in serum of summer that were LDH4 and LDH5 and one band in serum of autumn that was LDH5.The activities of LDH in tissues of skeleton muscle,cardiac muscle and brain were higher compared with the other tissues,it decreased markedly from spring to summer to autumn.In tissues of liver,kidney and lungs,activities of LDH were lower.Activities of LDH in livers,were significantly higher in spring compared that in summer and autumn,which had no obvious difference between summer and autumn.Activities of LDH in kidneys and lungs,showed no obviously difference between spring and summer,which decreased markedly in autumn.The spectrums of lactate dehydrogenase isoenzymes in tissues of cardiac muscle,li-ver,lungs,kidney,brain and skeleton muscle showed five bands,the spectrums were obvious different in different tissues,and the content of LDH isoenzymes showed seasonal changes in different tissues.Conclusion:Glycolysis levels in plateau zokors had obvious seasonally change which increased in spring and decreased in autumn significantly.It related to the activity of plateau zokors in diffe-rent seasons and seasonal fluctuation of oxygen and carbon dioxide in burrows of plateau zokors.