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白头翁汤治疗炎症性肠病的分子机制研究
作者姓名:Lu SW  Liu HJ  Zhao W  Li L  Dun HH  Liang C
作者单位:[1]成都中医药大学,四川成都610075 [2]中国航天员科研训练中心,北京100094
基金项目:国家自然科学基金面上项目(30871222); 军队医药卫生“十一五”科技攻关项目(06G002)
摘    要:目的:探讨白头翁汤治疗炎症性肠病的分子机制。方法:40只Wistar雄性大鼠随机分为5组(n=8):正常对照组、模型组、模型+阳性对照组(美沙拉嗪)、模型+中药治疗组,中药治疗组又分为中、高剂量组。阳性对照组、中药治疗组分别灌胃给药。疗程结束后取大鼠结肠组织利用免疫组织化学、Western blot等方法检测Smad7及p-Smad3在各组中的表达变化。结果:模型与组相比较,阳性药物及中药组尤其是高剂量组可有效抑制Smad7的表达,同时增强p-Smad3的表达。结论:白头翁汤可能通过激活TGF-β1/Smad3信号通路从而发挥了对炎症性肠病中的抗炎作用。

关 键 词:白头翁汤  炎症性肠病  转化生长因子  Smads

Molecular mechanisms involved in the treatment of inflammatory bowel disease by Pulsatilla decoction
Lu SW,Liu HJ,Zhao W,Li L,Dun HH,Liang C.Molecular mechanisms involved in the treatment of inflammatory bowel disease by Pulsatilla decoction[J].Chinese Journal of Applied Physiology,2011,27(1):106-109.
Authors:Lu Shu-Wen  Liu Hong-Ju  Zhao Wei  Li Li  Dun Huan-Huan  Liang Chao
Institution:Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Abstract:Objective: To investigate molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla decoction.Methods: Wistar male rats were randomly divided into control group,model group,model + positive control group(mesalazine),traditional Chinese medicine treatment group,in addition,the Chinese medical treatment group was divided into middle and high dose group(n=8).Intragastric administration was used in the positive control group and traditional Chinese medicine treatment group.The expression of Smad7 and p-Smad3 in the colons were detected by immunohistochemistry and Western blot.Results: Compared with the model group,positive medicine and traditional Chinese medicine group,especially high-dose group,could effectively inhibit the expression of Smad7,while enhancing the p-Smad3 expression.Conclusion: The activation of TGF-β1/Smad3 signaling pathway may be the molecular mechanism underlying in the anti-inflammatory effect of inflammatory bowel disease by Pulsatilla decoction.
Keywords:Pulsatilla decoction  inflammatory bowel disease  transforming growth factor  Smads
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