Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor |
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| Authors: | R G Hammonds R Schwall A Dudley L Berkemeier C Lai J Lee N Cunningham A H Reddi W I Wood A J Mason |
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| Institution: | Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080. |
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| Abstract: | The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo. |
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