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Chemotaxonomic Differentiation of Pinus Species Based on n-Alkane and Long-Chain Alcohol Profiles of Needle Cuticular Waxes |
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| Authors: | Prof Maria João Gaspar Dr Juliana Nunes Prof Miguel Rodrigues Prof Luis Ferreira |
| Institution: | 1. Center for the Research and Technology of Agro-Environmental and Biological sciences, University of Trás-os-Montes and Alto Douro (UTAD-CITAB) / Inov4Agro (Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production), Vila Real, Portugal
Center of Forestry Studies (CEF), ISA, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal;2. Center for the Research and Technology of Agro-Environmental and Biological sciences, University of Trás-os-Montes and Alto Douro (UTAD-CITAB) / Inov4Agro (Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production), Vila Real, Portugal |
| Abstract: | n-Alkane and long-chain alcohol (LCOH) profiles of needle cuticular waxes of nine provenances of three Pinus species (P. taeda. P. pinaster and P. pinea) were determined and their chemotaxonomic importance was studied. n-Alkanes concentrations were very low in all Pinus spp. A lack of ability of these compounds to differentiate Pinus spp. and their provenances was observed. LCOH concentrations were much higher, being nonacosan-10-ol (10-C29-OH) the most abundant one in all Pinus spp. Total LCOH concentrations varied (P<0.001) between Pinus spp. with P. taeda presenting the highest (P<0.05) value and P. pinea the lowest one. Differences in LCOH profiles were large (P<0.001) and allowed separation of Pinus spp. in the Principal Component Analysis (PCA). Although a noticeable separation of individuals was not observed. cluster analysis on LCOH concentrations allowed a clear distinction between species, indicating their potential to be used as chemotaxonomic markers to differentiate provenances of different Pinus spp. |
| Keywords: | n-alkanes long-chain alcohols Pinus chemotaxonomic markers provenances |