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Isolation and characterization of new microsatellites at the nm23-H1 and nm23-H2 gene loci and application for loss of heterozygosity (LOH) analysis |
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| Authors: | Markus Seifert Birgit Theisinger Matthias Engel Thomas Seib Gerhardt Seitz Manfred Stolte Karin Hilgert C Welter |
| Institution: | Institut für Humangenetik, Universit?t des Saarlandes, Geb?ude 68, D-66421 Homburg/Saar Germany, Tel.: +49 (0)6841166601, Fax: +49 (0)6841166600, e-mail: hgsdoo@med-rz.uni-sb.de, DE Institut für Pathologie, Klinikum Bamberg, D-96049 Bamberg, Germany, DE Institut für Pathologie, Klinikum Bayreuth, D-95445 Bayreuth, Germany, DE |
| Abstract: | The nm23-H1 gene has been suggested to be a metastasis suppressor gene. Studies about the events of loss of heterozygosity (LOH) at the nm23 locus and its correlation to metastasis are controversially discussed. To optimize detection of LOH at the nm23 locus, we screened two P1 clones for additional microsatellites. Tumor and normal DNA from 37 colorectal, 16 gastric, and 8 germ cancer patients were examined for LOH. We found two new CA repeats, one 5′ to nm23-H1 and another 3′ to nm23-H2. Using these nm23 locus-specific CA repeats and five other chromosome 17 loci (D17S1522, D17S1566, D17S855, D17S515, and TP53), allele loss was observed in 4/32 (12.5%) patients with colon cancer, 2/14 (14.3%) with gastric cancer, and 1/7 (14%) with germ cancer. No isolated LOH of the nm23 region was observed. Received: 5 May 1997 / Accepted: 2 June 1997 |
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