A rapid screening method for hepatocyte nuclear factor 1 alpha frameshift mutations; prevalence in maturity-onset diabetes of the young and late-onset non-insulin dependent diabetes |
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Authors: | Timothy M Frayling Michael P Bulman Maggie Appleton A T Hattersley Sian Ellard |
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Institution: | (1) Division of Molecular Genetics, Institute of Clinical Science, University of Exeter, Exeter, UK, GB |
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Abstract: | Non-insulin dependent diabetes (NIDDM) is a polygenic heterogeneous disorder of glucose homeostasis. Maturity-onset diabetes
of the young (MODY) is a monogenic subtype of NIDDM characterised by early-onset (< 25 years) and autosomal dominant inheritance.
Mutations in the hepatocyte nuclear factor 1 alpha (HNF-1α) gene have recently been shown to cause MODY. The incidence of
mutations in this gene in MODY and late-onset NIDDM is not known. We have developed a rapid specific polymerase chain reaction
test for HNF-1α mutations; this test involves the use of fluorescently labelled forward primers and modified reverse primers
to detect length polymorphisms resulting from frameshift mutations. With this method, we have screened 102 MODY probands,
viz. 60 defined according to strict diagnostic criteria (autosomal dominant inheritance and at least one member diagnosed
age < 25 years) and 95 late-onset NIDDM probands (diagnosed 35–70 years with ≥ 1 affected relative), for the presence of 9
known HNF-1α frameshift mutations, including 6 that occur at two sites for recurring mutation (residues 291/292 and 379).
Mutations were detected in 11 of the strictly defined MODY probands and one mutation was also found in a single subject with
early-onset NIDDM but no family history of the disease. The HNF-1α frameshift mutations were not detected in any late-onset
NIDDM subjects, suggesting these mutations do not have a major role in the pathogenesis of NIDDM. Our results indicate that
the prevalence of the nine frameshift mutations in strictly defined UK MODY is 18%, with the P291fsinsC mutation alone having
a frequency of 13%.
Received: 13 May 1997 / Accepted: 13 August 1997 |
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