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Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci |
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| Authors: | Jeffrey C Murray Rita Shiang Leon R Carlock Moyra Smith Kenneth H Buetow |
| Institution: | (1) Department of Pediatrics, University of Iowa Hospitals and Clinics, 52242 Iowa City, IA, USA;(2) Department of Pediatrics, University of California Irvine, 92717 Irvine, CA, USA;(3) Fox Chase Cancer Research Center, 19111 Philadelphia, PA, USA |
| Abstract: | Summary A rapid screening procedure for restriction fragment length polymorphisms (RFLPs) is reported. DNA from ten individuals is pooled and compared to DNA isolated from a cell line containing a single chromosome 4. This single chromosome-containing line is an obligate hemizygote for chromosome 4 RFLPs so that only one band corresponding to a single allele will appear on a Southern blot. In the pooled DNA sample lane bands corresponding to both alleles will be seen. The technique allows for efficient detection of RFLPs with easier use of large numbers of enzymes. It provides estimates of allele frequencies and disequilibrium. New RFLPs for albumin and alcohol dehydrogenase detected with this technique are described. |
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