A comparison of constitutive heterochromatin staining methods in two cases of familial heterochromatin deficiencies |
| |
Authors: | C H C M Buys G J P A Anders W L Gouw J M M Borkent-Ypma J A M Blenkers-Platter |
| |
Institution: | (1) Deparment of Human Genetics, State University of Groningen, Groningen, The Netherlands |
| |
Abstract: | Summary Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human chromosome 16 was indicated. The same C-banding procedure was also applied to a previously described familial deficiency of chromosome 9 heterochromatin evidenced using distamycin A/DAPI staining and G 11 staining (Buys et al., 1979). In this case a C-band appeared to be virtually absent on the relevant chromosome. These staining methods may be valuable tools in the study of chromosome polymorphisms. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|