| 肌动蛋白是扁绒泡菌Physarum compressum原质团细胞核核骨架成分 |
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| 引用本文： | 王晓丽,李艳双,盖玉红,李玉.肌动蛋白是扁绒泡菌Physarum compressum原质团细胞核核骨架成分[J].菌物学报,2010,29(2):185-187. |
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| 作者姓名： | 王晓丽 李艳双 盖玉红 李玉 |
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| 作者单位： | 吉林农业大学菌物所 长春 130118 |
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| 基金项目： | 国家自然科学基金（No. 30770005）；吉林农业大学博士启动基金（No. 203136） |
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| 摘 要： | 本研究以2mol/L NaCl或3, 5-二碘水杨酸锂（Lis）抽提扁绒泡菌Physarum compressum原质团细胞核，对残余蛋白进行电泳及肌动蛋白免疫印迹分析。结果表明单独用Lis抽提细胞核不能有效去除组蛋白，用2mol/L NaCl则可有效去除组蛋白，免疫印迹实验表明核骨架中存在着可以有效对抗2mol/L NaCl的肌动蛋白。
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| 关 键 词： | 黏菌，核基质，免疫印迹 |
Actin is one of the constituents of nuclear skeleton in plasmodia of Physarum compressum |
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| Authors: | WANG Xiao-Li LI Yan-Shuang GAI Yu-Hong LI Yu |
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| Institution: | Institute of Mycology, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Nuclear matrix residual protein was obtained by digesting and extracting Physarum compressum phaneroplasmodium nuclei,using 2mol/L NaCl and 3,5-diiodosalicylic acid lithium salt(Lis) respectively.SDS-PAGE analysis of residual matrix protein and Western blot analysis of actin indicated that the histons can't be removed by using Lis alone but can be removed by using 2mol/L NaCl.Actin in the nuclear matrix was resistant to 2mol/L NaCl effectively. |
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| Keywords: | myxomycetes nuclear matrix Western blot |
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