白腐真菌染料脱色菌株的筛选及一色齿毛菌脱色条件的研究

以平皿培养方式对采集自中国和芬兰的白腐真菌菌株降解6种不同结构的人工染料的能力进行了筛选研究。在40株菌株中,黑管孔菌Bjerkandera adusta Y5012,一色齿毛菌Cerrena unicolor Y5002,硬毛粗盖孔菌Funalia trogii Y4997,香栓孔菌Trametes suaveolens D8325和云芝栓孔菌Trametes versicolor Y4946对刚果红、橙黄G、茜素红、结晶紫、中性红和亚甲基蓝均显示出较强的脱色能力。对一色齿毛菌Cerrena unicolor Y5002的液体培养脱色条件进行了研究,其最适碳源和氮源分别为蔗糖和麦芽浸粉;在不同橙黄G浓度下均获得较高的脱色率,因此浓度为500mg/L的橙黄G未对该菌的脱色能力产生抑制作用,而浓度为400mg/L茜素红则对其脱色作用产生明显抑制。对菌丝生物量和染料脱色率的研究表明,在不同碳源和氮源条件下,两者之间具有明显的正相关性。

Screening of white-rot fungi for decolorizing synthetic dyes and investigation on factors affecting decolorizing capacity of Cerrena unicolor

The decolorization capacity of 40 different strains of white-rot fungi from China and Finland to 6 different synthetic dyes, Congo red, orange G, alizarin red, crystal violet, neutral red and methylene blue, were screened using agar plate tests. Among these strains, Bjerkandera adusta Y5012, Cerrena unicolor Y5002, Funalia trogii Y4997, Trametes suaveolens D8325 and Trametes versicolor Y4946 showed stronger capacity to decolorize the 6 different dyes. The factors related to decolorization of orange G and alizarin red of strain Cerrena unicolor Y5002 were studied by the method of shaking flask. Compared to the other carbon and nitrogen sources studied, sucrose and malt extract were found to be the suitable carbon/nitrogen sources. The decolorization efficiency of Cerrena unicolor Y5002 to orange G was similar at different orange G concentration below 500mg/L; however, alizarin red inhibited the decolorization reaction at the concentration of 400mg/L or above 400mg/L. The fungal biomass and dye decolorization had a positive relationship under the condition of different carbon and nitrogen sources.