| COI基因作为丛赤壳科真菌DNA条形码的测试 |
| |
| 引用本文： | 赵鹏,罗晶,庄文颖.COI基因作为丛赤壳科真菌DNA条形码的测试[J].菌物学报,2012,31(2):243-250. |
| |
| 作者姓名： | 赵鹏 罗晶 庄文颖 |
| |
| 作者单位： | 1. 中国科学院微生物研究所 真菌学国家重点实验室 北京100101;鲁东大学菌物科学与技术研究院山东省食用菌技术重点实验室 山东烟台264025
2. 中国科学院微生物研究所 真菌学国家重点实验室 北京100101 |
| |
| 基金项目： | 国家自然科学基金（No. 31070015）；中国科学院知识创新工程项目（No. KSCX2-EW-J-6） |
| |
| 摘 要： | 以丛赤壳科33种76个菌株为材料,探讨COI基因作为该科DNA条形码的可能性。结果表明,该DNA片段存在较多内含子,为了获取某些种的短片段,需设计许多引物,PCR扩增与测序成功率低,难以达到便捷、快速的物种鉴定的目的。因此,COI不宜作为丛赤壳科的DNA条形码。对已获得的少数片段进行分析表明,该基因对丛赤壳科部分种具有较强的物种鉴别力。
|
| 关 键 词： | 种内与种间差异 PCR扩增与测序成功率 邻接树 |
Can COI gene be used as DNA barcode for the nectriaceous fungi? |
| |
| Authors: | ZHAO Peng LUO Jing and ZHUANG Wen-Ying |
| |
| Institution: | State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Institute of Mycological Science and Technology and Shandong Province Key Laboratory of Edible Mushroom Technology, Ludong University, Yantai,;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| |
| Abstract: | COI gene was tested with 76 strains of 33 species of the nectriaceous fungi to investigate its feasibility of serving as a DNA barcode. The results indicated that introns are present in some species, which made it difficult for PCR amplification and sequencing. COI gene is not a good DNA barcode for this group. Analysis of the obtained COI sequences showed that this gene may have the capacity to distinguish species of the nectriaceous fungi. |
| |
| Keywords: | intra-and inter-specific variation PCR and sequencing success rate neighbor-joining tree |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
|
