微紫青霉菌Penicillium janthinellum菌株GXCR吸附废水中Cd2+的研究

研究了5种不同预处理方式对丝状真菌微紫青霉菌Penicillium janthinellum菌株 GXCR的Cd2+吸附的影响。结果表明，高温（80℃）、去离子水中的匀浆化、匀浆+碱化（NaOH，0.5mol/L）（简称匀浆碱化）和匀浆+30%二甲基亚砜处理均能提高菌体的吸附率，其中匀浆碱化处理后菌体的吸附效果最佳，吸附增量达到117.96%；匀浆+酸化（H2SO4， 0.5mol/L）处理则导致菌体的Cd2+吸附能力显著下降。匀浆碱化菌体吸附符合典型的Langmuir方程，表明该菌对Cd2+的吸附可能是以表面吸附为主的吸附行为。在吸附-解吸附循环4次后匀浆碱化菌体的Cd2+的吸附效率为58.01%。红外光谱分析显示匀浆碱化处理主要影响菌体表面分子的–OH和C=O基团，其中与Cd2+结合的主要基团是–OH。结果也表明，匀浆碱化菌体具有处理电镀废水的潜能。

Cd2+ biosorption in wastewater by Penicillium janthinellum strain GXCR

Effects of five pretreatment methods on Cd~(2+) biosorption of the mycelia of Penicillium janthinellum strain GXCR were investigated. The result showed that pretreatments of oven dry at 80℃, homogenate of mycelia in deionized water, basified homogenate of mycelia (NaOH, 0.5mol/L) and homogenate of mycelia in C_2H_6SO (30%) solution could enhance Cd~(2+) biosorption efficiency, in which pretreatment of basified homogenate was most effective, increasing the Cd~(2+) biosorption by 117.96%; acidified homogenate of mycelia in H_2SO_4 (0.5mol/L) led to significant decrease in Cd2+ bioadsorption capacity. Biosorption of basified homogenate of mycelia followed Langmuir isotherm equation, suggesting that the Cd2+ biosorption is surface sorption. After four sorption-desorption cycles, the basified homogenate of mycelia still had a Cd~(2+) biosorption efficiency of 58.01%. Inflated spectrometry analysis indicated that basified homogenate pretreatment might affect radicals of -OH and C=O on the mycelial surfaces, and the principal radicals linking Cd~(2+) was-OH. The results also indicated that basified homogenate of the mycelia might have a potential for the treatment of electroplating wastewater.