雷公藤解毒持效双向发酵工艺的建立

采用双向发酵的原理,运用"发酵过程动态比较法"研究了灵芝接种于雷公藤药性基质上发酵不同时间所得菌质的化学成分、急性毒性和免疫功能的变化,以其确定雷公藤解毒持效双向发酵的最佳发酵终点。研究结果表明:发酵第30天所得的菌质(G30)总二萜的含量最低,为0.57%;与雷公藤生药比较,G30的LD50最高,且发酵第30天所得菌质的体液免疫和细胞免疫抑制作用最强。综合对成分含量、毒性及药效的动态数据联系比较、分析,确定了雷公藤解毒持效双向发酵的发酵终点与适宜的发酵周期是菌丝长满瓶后的第30天。

Optimization of bi-direction solid fermentation for toxicity- reducing and efficacy-maintaining action of fungal fermentative products of Tripterygium wilfordii

Tripterygium wilfordii has been traditionally used as folk medicine for centuries in China for the treatment of immune-inflammatory diseases. However, Tripterygium wilfordii has a narrow therapeutic window. In order to increase the therapeutic index of Tripterygium wilfordii, a novel fungal fermentative method, i.e. bi-direction solid fermentation was used. Our previous studies have demonstrated that the medicinal mushroom Ganoderma lucidum has a good toxicity-reducing efficacy and efficacy-maintaining action. The present study aims at establishing the technology of toxicity-reducing and efficacy-maintaining action of fungal fermentative products of Tripterygium wilfordii by using bi-direction solid fermentation method and finding the optimum fermenting condition. Dynamic comparison in the course of fermentation using Ganoderma lucidum as inoculum was conducted, and the changes of chemical composition, acute toxicity and immunologic function of Ganoderma lucidum-Tripterygium wilfordii combination in different fermentative duration were observed. The results showed that the content of total diterpene was the lowest (only 0.57%) in the fungal fermented substance (G30) in incubation period of 30 days. Compared with the crude drug of Tripterygium wilfordii, G30 had the highest LD50 and the best effects of immunosuppression both in humoral immunity and cellular immunity. Synthetical analysis showed that, fermentative period of 30 days could be regarded as optimum for toxicity-reducing and efficacy-maintaining action of the fungal fermentative products in Tripterygium wilfordii by bi-direction solid fermentation.