玉米培养物中弯角镰孢菌代谢产物的分析与鉴定

从致牛烂蹄病的稻草中分离的弯角镰孢菌菌株(Fusarium camptoceras)于1988年和1991年分两批接种玉米中,培养物用乙腈:水(3:1,v/v)提取,正已烷脱脂,Florisil色谱柱净化,经净化的提取液经电子捕获气相色谱(GC-ECD)、薄层色谱(TLC)分析和气相色谱-质谱联用(GC-MS)鉴定,证实了弯角镰孢菌可同时产生多种单端孢霉烯族化合物。弯角镰孢菌能同时产生这些代谢物,在国内外未见报道。

ANALYSIS AND IDENTIFICATION OF FUSARIUM MYCOTOXIONS IN CORN CULTURE OF FUSARIUM CAMPTOCERAS

Corn cultures of Fusarium camptoceras isolated from the rice straw causing the disease of Fescue foot were analysed with TLC, GE-ECD and GC-MS. Several trichothecenes including nivalenol (NIV), fusarenon-X (F-X), scirpentrol (SCP), 7-deoxynivalenol (7-DON), 3-acetoxyscirpentriol (3-ASCP), 4-acetoxyscirpentriol (4-ASCP), 3-acetyldeoxynivalenol (3-ADON), 7-acetyldeoxynivalenol (7-ADON), 4,15 diacetylnivalenol (4,15-DANIV), 7,8-dihydroxydiacetoxyscirpenol (DOHDAS), etc. had been identified by GC-MS. No butenolide and zearalenone were found in these cultures. This is the first report about these (?)richothecenes produced by Fusarium camptocern. These trichothecenes may be a caustive factor of the disease of fescue foot.