两株古尼拟青霉菌株的镇痛差异蛋白质组学研究

为了探明古尼虫草无性型--古尼拟青霉差异表达蛋白与镇痛活性之间的关系,对有镇痛活性和无镇痛活性的两个古尼拟青霉菌株,进行蛋白质差异表达研究,结果显示,活性菌株(HZJ-1)中出现或表达显著上调的蛋白质点数为51个,结构鉴定得到39个类别功能各异的蛋白质,其中大量蛋白与代谢相关,其中Nhd3P特异性谷氨酸脱氢酶可能参与使...

Differential expression of analgesic proteins in two Paecilomyces gunnii isolates

Two isolates of Paecilomyces gunnii, anamorph of Cordyceps gunnii, with and without producing analgesic activity, were tested by using proteomic analysis in order to find the correlation between protein differential expression and analgesic activity. The results showed that expression of 51 protein spots significantly increased in the active isolate HZJ-1. Among the spots, structures of 39 proteins with various functions were identified. Most of the proteins were related to metabolism, and the NADP-specific glutamate dehydrogenase might mediate long-term depression of synaptic transmission and reduce pain. The functions of several conserved hypothetical proteins have not been reported, and are needed for further studies.