| 鲍姆木层孔菌多糖对HepG2细胞增殖及侵袭相关能力的抑制作用 |
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| 引用本文： | 王贵宾,董露璐,姬媛媛,相文华,赵敏.鲍姆木层孔菌多糖对HepG2细胞增殖及侵袭相关能力的抑制作用[J].菌物学报,2011,30(2):288-294. |
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| 作者姓名： | 王贵宾 董露璐 姬媛媛 相文华 赵敏 |
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| 作者单位： | 1. 东北林业大学生命科学学院,哈尔滨,150040
2. 中国农业科学院哈尔滨兽医研究所,哈尔滨,150001 |
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| 摘 要： | 证实了鲍姆木层孔菌多糖对肝癌细胞系HepG2细胞增殖及侵袭的抑制作用。鲍姆木层孔菌提取物经乙醇分级沉淀和DEAE-Sepharoes F.F.离子柱层析以及Sephacryl S-200凝胶柱层析纯化，获得均一多糖PLP60-B1，该多糖具有体外活性。MTT法及流式细胞仪技术证实多糖PLP60-B1通过致使HepG2细胞阻滞于S期而显著抑制HepG2细胞的增殖和细胞集落的形成，且也可显著抑制细胞的粘附及侵袭能力。
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| 关 键 词： | 大型真菌 抗肿瘤 细胞周期 侵袭 |
Inhibitory effects of Phellinus baumii polysaccharide on the proliferation and invasion of HepG2 cell |
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| Authors: | WANG Gui-Bin DONG Lu-Lu JI Yuan-Yuan XIANG Wen-Hua and ZHAO Min |
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| Institution: | College of Life Sciences, Northeast Forestry University, Harbin 150040, China;College of Life Sciences, Northeast Forestry University, Harbin 150040, China;Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;College of Life Sciences, Northeast Forestry University, Harbin 150040, China |
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| Abstract: | The inhibitory effects of Phellinus baumii polysaccharide PLP60-B1 on the proliferation and invasion of HepG2 cancer cells were investigated. The homogeneous Phellinus baumii polysaccharide PLP60-B1 having promoting effects on lymphocyte proliferation was obtained by ethanol precipitation and purified by DEAE-Sepharose F.F. and Sephacryl S-200. MTT assay and flow cytometry showed that PLP60-B1 inhibited cell proliferation as well as colony formation of HepG2 cells through the S-phase cell cycle arrest, and the homogeneous polysaccharide also demonstrated the remarkably inhibition of adhesion and invasion in HepG2 cells. |
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| Keywords: | macrofungi antitumor cell cycle invasiveness |
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