| 一种六妹羊肚菌的新型柄腐病害 |
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| 引用本文： | 刘天海,周洁,王迪,何晓兰,唐杰,陈影,王嘉,彭卫红.一种六妹羊肚菌的新型柄腐病害[J].菌物学报,2021,40(9):2229-2243. |
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| 作者姓名： | 刘天海 周洁 王迪 何晓兰 唐杰 陈影 王嘉 彭卫红 |
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| 作者单位： | 1.四川省农业科学院农业资源与环境研究所 四川 成都 6100662.四川省农业科学院食用菌研究中心 四川 成都 6100663.四川省农业科学院 四川 成都 610066 |
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| 基金项目： | 四川食用菌创新团队(SCCXTD-2021-07);国家食用菌产业技术体系(CARS-20) |
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| 摘 要： | 近年来,四川等地种植的六妹羊肚菌先后爆发柄腐病害,造成严重经济损失。本研究对该病害发生情况进行了调查,从四川、河南、甘肃、贵州4个省收集的12份六妹羊肚菌柄腐病子实体,分离纯化得到37株真菌和3株细菌。根据柯赫氏法则,对所有分离纯化得到的疑似病原菌进行了回接试验,最终确定GJB-3菌株为柄腐病的病原菌。经ITS、RPB2和EF1α序列分析,结合菌落形态和显微特征鉴定柄腐病的病原菌为Fusarium nematophilum。本研究为后期羊肚菌生产病害防控提供了有效的基础数据。
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| 关 键 词： | 食用菌病害 镰刀菌 柯赫氏法则 分子鉴定 |
| 收稿时间： | 2021-02-02 |
A new stipe rot disease of the cultivated Morchella sextelata |
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| Authors: | LIU Tian-Hai ZHOU Jie WANG Di HE Xiao-Lan TANG Jie CHEN Ying WANG Jia PENG Wei-Hong |
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| Institution: | 1. Institute of Agricultural Resources and Environment, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China2. Sichuan Academy of Agricultural Sciences Mushroom Research Center, Chengdu, Sichuan 610066, China3. Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066, China |
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| Abstract: | In recent years, stipe rot disease caused heavy losses in Morchella sextelata cultivation. In order to clarify the pathogen, 12 samples of diseased Morchella sextelata fruiting bodies from Sichuan, Henan, Gansu, and Guizhou were collected. Thirty-seven fungal strains and three bacterial strains were isolated from these samples. According to the Koch’s rule, GJB-3 was confirmed as the pathogenic strain. Based on ITS, RPB2 and EF1α sequences analyses, colony morphology and microscopic structures, GJB-3 was identified as Fusarium nematophilum. Prevention and control of stipe rot disease on Morchella will be further studied. |
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| Keywords: | edible mushroom disease Fusarium Koch’s rule molecular identification |
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