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咖啡种子中的内生真菌
作者姓名:Fernando E. VEGA  Francisco POSADA  M. Catherine AIME  Stephen W. PETERSON  Stephen A. REHNER
作者单位:Fernando E. VEGA(Sustainable Perennial Crops Laboratory, U. S. Department of Agriculture, Agricultural Research Service, Building 011A, BARC-W, Beltsville, Maryland 20705, USA)  Francisco POSADA(Centro Nacional de Investigaciones de Café, CENICAF(E), Chinchiná, Caldas, Colombia)  M. Catherine AIME(Department of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, 302 Life Sciences Bldg., Baton Rouge, LA 70803, USA)  Stephen W. PETERSON(Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, U. S.Department of Agriculture, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA)  Stephen A. REHNER(Systematic Mycology and Microbiology Laboratory, U. S. Department of Agriculture, Agricultural Research Service, Building 011A, BARC-W,, Beltsville, Maryland 20705, USA)
摘    要:Green coffee seeds from Colombia, Guatemala, India, Kenya, Papua New Guinea, Puerto Rico and Vietnam were sampled for the presence of fungal endophytes. Stions of surface sterilized seeds were plated on yeast malt agar, and fungal growth was isolated for subsequent DNA extraction and sequencing. Several fungal genera were isolated, including Acremonium, Aspergillus, Eurotium, Fusarium, Gibberella, Penicillium, Pseudozyma and an undescribed clavicipitaceous species. The biological activities that these fungi might be playing in coffee seeds remain unknown, but in other plants some of the genera isolated have been reported to protect against plant pathogens.

修稿时间:2007年8月29日

Fungal endophytes in green coffee seeds
Authors:Fernando E VEGA  Francisco POSADA  M Catherine AIME  Stephen W PETERSON and Stephen A REHNER
Institution:1. Sustainable Perennial Crops Laboratory, U. S. Department of Agriculture, Agricultural Research Service, Building 011A, BARC-W, Beltsville, Maryland 20705, USA
2. Centro Nacional de Investigaciones de Café, CENICAF(E), Chinchiná, Caldas, Colombia
3. Department of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, 302 Life Sciences Bldg., Baton Rouge, LA 70803, USA
4. Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, U. S.Department of Agriculture, Agricultural Research Service, 1815 N. University St., Peoria, IL 61604, USA
5. Systematic Mycology and Microbiology Laboratory, U. S. Department of Agriculture, Agricultural Research Service, Building 011A, BARC-W,, Beltsville, Maryland 20705, USA
Abstract:Green coffee seeds from Colombia, Guatemala, India, Kenya, Papua New Guinea, Puerto Rico and Vietnam were sampled for the presence of fungal endophytes. Stions of surface sterilized seeds were plated on yeast malt agar, and fungal growth was isolated for subsequent DNA extraction and sequencing. Several fungal genera were isolated, including Acremonium, Aspergillus, Eurotium, Fusarium, Gibberella, Penicillium,Pseudozyma and an undescribed clavicipitaceous species. The biological activities that these fungi might be playing in coffee seeds remain unknown, but in other plants some of the genera isolated have been reported to protect against plant pathogens.
Keywords:Acremonium  Aspergillus  Clavicipitaceae  Coffea  endophytic  Penicillium  Pseudozyma
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