草菇子实体多肽提取工艺及其抗氧化活性

为了优化草菇子实体多肽的提取工艺和探究其抗氧化活性，以草菇子实体为原料，采用酶解法提取草菇子实体多肽，通过单因素试验得出最佳的酶解工艺，并使用Box-Behnken设计试验组合。结果表明：草菇子实体提取多肽的最佳工艺为料液比1:52 (g/mL)、加酶量7 200 U/g、酶解温度43 ℃，此工艺条件下的多肽得率为67.76%。从1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、铁离子还原能力、超氧阴离子自由基清除能力和羟自由基清除能力4个方面研究其体外抗氧化能力，结果表明，草菇子实体多肽对DPPH自由基清除率为74.11%，超氧阴离子自由基和羟自由基清除率分别在69.64%和91.83%达到稳定，草菇子实体多肽还具有一定的还原力，说明草菇子实体多肽可以作为优质抗氧化肽的良好来源。该研究为草菇多肽的高效制备和抗氧化肽等高附加值产品的研发提供理论依据。

Extraction technology and antioxidant activities of polypeptides from fruiting bodies of Volvariella volvacea

Extraction process of Volvariella volvacea fruiting body polypeptide is optimized, and antioxidant activities of the polypeptide is explored. The polypeptide of fruiting bodies was extracted by enzymatic hydrolysis. The enzymatic hydrolysis process was optimized by single factor experiment, and the combination of experiments was designed using Box-Behnken. The results showed that the optimal process for extracting polypeptide from V. volvacea fruiting bodies was as follows: the ratio of solid to liquid was 1:52 (g/mL), the amount of enzyme added was 7 200 U/g, and the enzymatic hydrolysis temperature was 43 °C. The yield of polypeptide under these process conditions was 67.76%. The antioxidant capacity in vitro was studied on the basis of DPPH free radical scavenging ability, iron ion reducing ability, superoxide anion free radical scavenging ability and hydroxyl free radical scavenging ability. The results showed that the DPPH radical clearance rate was 74.11%, and superoxide anion free radical and hydroxyl free radical scavenging rates were stably 69.64% and 91.83%, respectively. V. volvacea fruiting body polypeptide also has reducing power to a certain extent, indicating that V. volvacea fruiting body polypeptide can be regarded as a good source of high-quality antioxidant peptides. It is hopeful that V. volvacea peptides could be developed as high-added value products such as antioxidant peptides.