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四川主要松树内生拟盘多毛孢
引用本文:刘韩,王欢,赵景阳,袁川,李万艳,刘应高.四川主要松树内生拟盘多毛孢[J].菌物学报,2013,32(1):15-24.
作者姓名:刘韩  王欢  赵景阳  袁川  李万艳  刘应高
作者单位:1. 四川农业大学林学院 四川雅安625014;四川省甘孜州林业科学研究所 四川康定626001
2. 四川农业大学林学院 四川雅安625014
基金项目:四川省教育厅重点项目(No. 09ZA068)
摘    要:从5种松树健康的枝、叶、干中共分离了2,400个组织块,得到199株内生拟盘多毛孢Pestalotiopsis,形态学和分子生物学鉴定分属于11个种,平均分离率为8.29%。所得11种内生拟盘多毛孢都是首次在四川的松属植物中分离得到,其中葡萄生拟盘多毛孢P.uvicola和山桃草拟盘多毛孢P.gaurae为首次在松树上分离得到。基于ITS序列的分子系统发育分析显示松树内生拟盘多毛孢种类之间存在一定的遗传差异,部分内生拟盘多毛孢与病原拟盘多毛孢的ITS序列具有很高的同源性。

关 键 词:拟盘多毛孢属  形态特征  内转录间隔区序列

Endophytic Pestalotiopsis from the main pine species in Sichuan
Authors:LIU Han  WANG Huan  ZHAO Jing-Yang  YUAN Chuan  LI Wan-Yan and LIU Ying-Gao
Institution:1 College of Forestry, Sichuan Agricultural University, Ya’an, Sichuan 625014, China 2 Ganzi Institute of Forestry Research, Kangding, Sichuan 626001, China
Abstract:One hundred and ninety-nine isolates of endophytic Pestalotiopsis were isolated from 2,400 tissue masses of healthy branches, leaves and stems of five species of pine in Sichuan, and 11 species were identified based on morphological characteristics and ITS sequences. The average isolation rates of endophytic Pestalotiopsis was 8.29%. P. uvicola and P. gaurae were dominant among the 11 species, which were firstly recorded on pines in Sichuan. Molecular phylogenetic analysis based on ITS sequences indicated that there were some genetic differences among these endophytic Pestalotiopsis species, and part of them were highly homologeneous with the pathogenic Pestalotiopsis.
Keywords:Pestalotiopsis  morphological characteristics  ITS sequences
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