固态混合发酵提高木聚糖酶和纤维素酶活力的研究

研究了接种比例、接种时间、碳源、氮源等因素对木霉和黑曲霉混合发酵产木聚糖酶和纤维素酶的影响。试验结果表明,当木霉和黑曲霉按4:6同时接种,以玉米芯3．75g、麸皮3．75g、葡萄糖37．5mg为混合碳源,Mandels营养盐11．5mL、添加NH_4NO_37．5mg为氮源,在84h产纤维素酶活力达到230IU/g干物质,木聚糖酶活力达到1308IU/g干物质,与两菌纯培养相比,纤维素酶活力提高163％,木聚糖酶活力提高79．5％。

Solid-state mixed fermentation increases activities of xylanase and cellulase

The influences of different factors,such as inoculum proportion,different incubation time,carbon sources and nitrogen sources etc.,on the activities of xylanase and cellulase produced by solid-state mixed fermentation of Aspergillus niger A3 and Trichoderma sp.T6 were studied.The result showed that the optimal conditions for solid-state mixed fermentation of T6 and A3 was as follows:mixed inoculation of T6 and A3 in the ratio of 4:6,mixed carbon sources containing 3.75g corn cob,3.75g wheat bran and 37.5mg glucose,and 11.5mL Mandels nutrient solution containing 7.5mg NH_4NO_3.In the period of 84h,mixed fermentation CMCase activity reached 230IU/g dry matter and increased by 163%,and xylanase activity reached 1308IU/g dry matter and increased by 79.5% over solitary fermentation of T6 and A3.