| 应用5.8S rDNA及ITS区序列分析链格孢种级分类* |
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| 引用本文： | 王洪凯,张天宇,张猛.应用5.8S rDNA及ITS区序列分析链格孢种级分类*[J].菌物学报,2001,20(2). |
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| 作者姓名： | 王洪凯 张天宇 张猛 |
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| 作者单位： | 中国科学院真菌地衣系统学开放实验室；山东农业大学植病系；浙江大学生物技术研究所 |
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| 摘 要： | 对9个链格孢小孢子种和3个大孢子种共20个链格孢菌株的5.8S rDNA及其两侧的ITS1区和ITS2区进行了序列分析。聚类分析结果表明形态差异大的种可以明确加以区分，而供试的9个小孢子种之间差异很小，不能根据对所选区段的序列分析加以区分。传统分类上的滨菊链格孢不属于Alternaria, 其分类地位需进一步研究。
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| 关 键 词： | 链格孢 序列分析 ITS区 rDNA5.8s段 |
APPLICATION OF SEQUENCING OF 5.8S rDNA,ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL |
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| Abstract: | ： The sequences coding for the 5.8S rDNA and the flanking internal transcribed spacers (ITS1 and ITS2) were amplified by PCR and sequenced from 9 small-spored species and other 3 large-species in Alternaria. Phylogenetic analysis clearly separated the 2 large-spored species from each other and from 9 small-spored species tested, but small-spore species cannot be clearly separated each other. This indicate that small-spored species tested belong to a natural species group. Traditional taxonomic morphologically distinguished species A.leucanthemi may not belong to Alternaria according to sequence analysis, its taxonomic position require further study. |
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| Keywords: | Alternaria， sequence analysis，5 8S rDNA， ITS spacers |
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