| 丙酮提取金顶侧耳发酵液多糖的优化设计 |
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| 引用本文： | 闫训友,李娜娜,史振霞,王玮,吴智艳.丙酮提取金顶侧耳发酵液多糖的优化设计[J].菌物学报,2008,27(3):413-419. |
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| 作者姓名： | 闫训友 李娜娜 史振霞 王玮 吴智艳 |
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| 作者单位： | 廊坊师范学院生命科学学院,廊坊,065000 |
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| 基金项目： | 廊坊市科学技术研究与发展计划项目
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河北省第二批科学技术研究与发展指导计划 |
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| 摘 要： | 以液体深层发酵法培养金顶侧耳,发酵液以丙酮为提取剂在不同的浓度﹑时间﹑温度条件下提取多糖,利用正交设计试验分析,得到了一种优化方案。试验结果表明:发酵液多糖提取最优方案是提取温度为70℃、丙酮浓度为75%、提取时间为1.5h。
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| 关 键 词： | 金顶侧耳 发酵液浓度 提取时间 提取温度 正交试验 |
Optimum extraction process of polysaccharide from Pleurotus citrinopileatus fermentation liquid by acetone |
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| Authors: | YAN Xun-You LI Na-N SHI Zhen-Xi WANG Wei WU Zhi-Yan |
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| Institution: | College of Life Science, Langfang Teachers College, Langfang 065000, China |
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| Abstract: | Pleurotus citrinopileatus polysaccharides ware extracted from fermentation liquid by acetone under the conditions of different solvent concentration, extracting duration and temperature. The optimal design of extraction was analysed by using orthogonal test. The results showed that under the condition of acetone concentration of 75%, extraction duration of 1.5h, and temperature of 70℃, the highest yield of Pleurotus citrinopileatus polysaccharides was obtained. |
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| Keywords: | Pleurotus citrinopileatus concentration extraction duration temperature orthogonal test |
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