两株地理隔离的桦褐孔菌深层发酵产生抗氧化次生代谢产物的代谢组学分析

采用基于核磁共振代谢组学的方法研究了两株地理隔离的桦褐孔菌中国牡丹江菌株(IOM)和芬兰万塔菌株(IOV)在深层培养过程中主要代谢产物组成的差异。IOM和IOV两菌株在培养过程中积累菌丝体生物量、菌丝体酚类(TMP)以及三萜化合物(TMT)的过程相似,但IOM菌株产生较高水平的TMP。代谢产物组成动力学比较结果表明,IOM在第3天含有较高水平的硬毛素类似物、桦褐孔菌二糖和TMT,但在第5天均有所下降。导致这些差异的代谢产物为fuscoparianolD,21,24-羊毛甾-7,9(11),23-三烯-3β,22,25-三醇,羊毛甾-8,23-二烯-3β,22,25-三醇,23,24-环戊羊毛甾-3β,21,25-三醇-8-烯,桦褐孔菌二糖,phelligridinsC,D及H,甲基地花菌素A和C,地花菌素B,davallialactone及methyl davallialactone。这些代谢产物在IOV的提取物中均参与清除自由基,而在IOM的提取物中主要是酚类化合物参与自由基的清除。两菌株的这些差异反应了环境条件对桦褐孔菌生长及代谢的影响,即不仅影响其次生代谢产物的合成,还影响代谢产物在防止细胞氧化损伤过程中所起的作用。

Metabonomic analysis on production of antioxidant secondary metabolites by two geographically isolated strains of Inonotus obliquus in submerged cultures

This study evaluated metabolic discrepancies between two geographically isolated cultures of Inonotus obliquus from Mudanjiang (IOM) and Vantaa (IOV) by NMR-based metabonomic analysis. IOM and IOV both accumulated mycelia, total mycelial phenolic compounds (TMP) and triterpenoids (TMT) in similar patterns, but with higher maximum TMP levels produced by IOM. Comparisons of culture kinetics of metabolites showed that higher amounts of detected hispidin analogs, inotodisaccharide and TMT were produced in IOM at day 3, but these levels fell from day 5. The metabolites responsible for these differences were fuscoparianol D, 21,24-lanosta-7,9(11),23-triene-3b,22,25-triol, lanosta-8,23-diene-3b,22,25-triol, 23,24-cyclopentalanosta-3b,21,25-triol-8-en, inotodisaccharide, phelligridins C and D and H, methylinoscavins A and C, inoscavins B, davallialactone and methyldavallialactone. These metabolites in IOV are all involved in scavenging free radicals, whereas those in IOM are phenolics. These differences between the two strains reflect different environmental conditions under which each is grown, affecting not only secondary metabolite biosynthesis, but their roles in protecting cells from oxidative damagea as well.