| 淡黄绒泡菌原质团的培养及其rDNA-ITS序列分析 |
| |
| 引用本文： | 潘景芝,张旭,刘福杰,王琦,李玉.淡黄绒泡菌原质团的培养及其rDNA-ITS序列分析[J].菌物学报,2010,29(6):893-896. |
| |
| 作者姓名： | 潘景芝 张旭 刘福杰 王琦 李玉 |
| |
| 作者单位： | 吉林农业大学食药用菌教育部工程研究中心,长春,130118 |
| |
| 基金项目： | National Nature Science Foundation of China (No. 30670015) |
| |
| 摘 要： | 首次实现了黏菌淡黄绒泡菌Physarum melleum原质团的燕麦琼脂培养，并通过ITS引物完成了淡黄绒泡菌rDNA的PCR扩增及序列分析。描述了其原质团的培养方法，提供了原质团rDNA的提取方法及ITS引物的扩增条件。利用邻近结合法构建系统发育树，对淡黄绒泡菌与相关分类单元的分子系统学关系进行了初步探讨。
|
| 关 键 词： | 培养条件，PCR扩增，系统发育 |
Oat-agar culture and rDNA-ITS sequence analyses of the plasmodium of Physarum melleum in Myxomycetes |
| |
| Authors: | PAN Jing-Zhi ZHANG Xu LIU Fu-Jie WANG Qi and LI Yu |
| |
| Institution: | Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China |
| |
| Abstract: | Plasmodium of Physarum melleum in myxomycetes was cultured in oat-agar medium, and rDNA-ITS from the plasmodia was amplified and sequenced. The culture condition of the plasmodia was described. DNA preparation method and PCR amplification conditions from the plasmodia were provided. The molecular phylogenetic relationships among P. melleum and related species were analyzed based on rDNA-ITS sequences using Neighbor-joining method. |
| |
| Keywords: | culture condition PCR amplification phylogenetic |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
|
