| 牛肝菌属卷边组Boletus sect. Appendiculati物种的分子识别 |
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| 引用本文： | 魏海龙,李海波,王丽玲,丁红梅,胡传久,曾凡清,程俊文.牛肝菌属卷边组Boletus sect. Appendiculati物种的分子识别[J].菌物学报,2014,33(2):242-253. |
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| 作者姓名： | 魏海龙 李海波 王丽玲 丁红梅 胡传久 曾凡清 程俊文 |
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| 作者单位： | 浙江省林业科学研究院 浙江 杭州 310023;浙江省林业科学研究院 浙江 杭州 310023;浙江省林业科学研究院 浙江 杭州 310023;浙江中医药大学 浙江 杭州 310053;浙江省林业科学研究院 浙江 杭州 310023;丽水市农业科学研究院 浙江 丽水 323000;浙江省林业科学研究院 浙江 杭州 310023 |
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| 基金项目： | 浙江省自然科学基金（No. Y3100345，Y3090380，Y3110048）；浙江省森林食品研究创新团队建设与人才培养项目（No. 2012F20012）；丽水市科技计划项目（No. 20120410） |
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| 摘 要： | 依据牛肝菌属卷边组Boletus sect. Appendiculati内7个物种的核糖体基因rDNA内转录间隔区（internal transcribed spacer，ITS）序列比对，设计5对ITS特异引物，分别用于卷边牛肝菌Boletus appendiculatus与亚卷边牛肝菌B. subappendiculatus、卷边牛肝菌与拟桃红牛肝菌B. pseudoregius、华靛牛肝菌B. roseoflavus与卷边牛肝菌B.?appendiculatus、华靛牛肝菌与华美牛肝菌B. speciosus、拟桃红牛肝菌与华美牛肝菌的相互识别。ITS区段的PCR扩增结果表明，5对ITS特异引物皆成功扩增出可用于辨别这些近缘物种的目的条带。但未能设计出ITS特异引物，以识别华靛牛肝菌与桃红牛肝菌B. regius两个近缘物种。
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| 关 键 词： | 牛肝菌 分类 ITS |
Molecular recognition of species in Boletus sect. Appendiculati |
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| Authors: | WEI Hai-Long LI Hai-Bo WANG Li-Ling DING Hong-Mei HU Chuan-Jiu ZENG Fan-Qing and CHENG Jun-Wen |
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| Institution: | Zhejiang Forestry Academy, Hangzhou, Zhejiang 310023, China;Zhejiang Forestry Academy, Hangzhou, Zhejiang 310023, China;Zhejiang Forestry Academy, Hangzhou, Zhejiang 310023, China;Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China;Zhejiang Forestry Academy, Hangzhou, Zhejiang 310023, China;Lishui Academy of Agricultural Sciences, Lishui, Zhejiang 323000, China;Zhejiang Forestry Academy, Hangzhou, Zhejiang 310023, China |
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| Abstract: | Based on the sequences of internal transcribed spacer (ITS) of seven species in Boletus sect. Appendiculati, five pairs of specific ITS primers were designed and used for the amplification of ITS region in order to distinguish B. appendiculatus versus B. subappendiculatus, B. appendiculatus versus B. pseudoregius, B. roseoflavus versus B. appendiculatus, B. roseoflavus versus B. speciosus, and B. pseudoregius versus B. speciosus, respectively. The results of PCR analysis using the five specific ITS primers showed that the target band is amplified successfully from the species pairs, respectively, which could be used for the molecular recognition of these species. However, the molecular recognition of B. roseoflavus versus B. regius is not achieved for lacking specific ITS primers to be designed. |
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| Keywords: | Boletus taxonomy ITS |
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