黑里河和贺兰山自然保护区华北落叶松根区土壤中外生菌根真菌群落

为了解华北落叶松外生菌根（ectomycorrhiza,ECM）真菌的群落组成及探究土壤理化性质对其造成的影响,本研究选取内蒙古黑里河自然保护区道须沟与大坝沟以及贺兰山自然保护区南寺,采集华北落叶松林下根区土壤,利用Illumina MiSeq测序平台对真菌DNA扩增子ITS1区进行高通量测序。共获得ECM真菌序列65 859条,划分为436个OTU,隶属于2门6纲12目26科41属,主要分布于担子菌门Basidiomycota。3个采样地共有优势ECM真菌OTU 17个,归属于红菇属Russula、丝膜菌属Cortinarius、阿太菌属Amphinema、Piloderma、锁瑚菌属Clavulina、丝盖伞属Inocybe、空团菌属Cenococcum、棉革菌属Tomentella和Meliniomyces;黑里河自然保护区道须沟与大坝沟的根区土壤ECM真菌群落组成相似：热图表明两地在ECM真菌属水平的组成方面相似,PerMANOVA分析表明两地群落组成差异不显著,而与贺兰山自然保护区南寺存在显著差异（P=0.0234）,优势ECM真菌的多样性与丰度都较南寺低;土壤理化性质TP与pH产生的协同作用与采样地的ECM真菌群落组成具有显著相关性（P=0.0383）,对南寺华北落叶松ECM真菌群落组成造成正相关影响,对道须沟与大坝沟造成负相关影响。

Ectomycorrhizal fungal community in the root zone soil of Larix gmelinii var. principis-rupprechtii in Heilihe and Helanshan National Nature Reserve

The ectomycorrhizal(ECM) fungal community composition in the root zone soilof Larix gmelinii var. principis-rupprechtii in Inner Mongolia was investigated, and the effect of soil physicochemical properties on the community was explored. Daoxugou and Dabagou of Heilihe National Nature Reserve, and Nansi of Helanshan National Nature Reserve were selected as sample plots. DNA ITS1 region of the fungi in root zone soil were sequenced using the Illumina MiSeq High-throughput sequencing platform. A total of 65 859 sequences of ECM fungi obtained, were divided into 436 OTUs which belong to two phyla, six classes, 12 orders, 26 families and 41 genera, being mainly distributed in Basidiomycota. The dominant ECM fungal OTUs totalled 17, belonging to Russula,Cortinarius,Amphinema,Piloderma,Clavulina,Inocybe,Cenococcum,Tomentella and Meliniomyces. The fungal community composition in Daoxugou and Dabagou of Heilihe National Nature Reserve are similar. The heatmap indicates both localities are similar in composition at genus level. PerMANOVA shows that the difference of composition between the two localities is not significant. However, ECM fungal community composition in the both localities is significantly different from that in Nansi of Helanshan National Nature Reserve (P=0.0234), and the diversity and abundance of dominant ECM fungi are lower than those in Nansi. The synergetic effect of soil physicochemical propertiesTP and pH has significant relation (P=0.0383)to ECM fungal community composition, showing positive effect on the ECM fungi community of L. gmelinii var. principis-rupprechti in Nansi, and negative effect on that in Daoxugou and Dabagou.