球毛壳菌H6次级代谢产物及其α-葡萄糖苷酶抑制活性

采用体外α-葡萄糖苷酶抑制模型对一株球毛壳菌H6的发酵液和菌丝体两种乙酸乙酯提取物进行活性评价,结果表明,发酵液乙酸乙酯提取物对α-葡萄糖苷酶具有较强的抑制活性,其IC₅₀值为(510.76±23.46)μg/mL。采用硅胶、Sephadex LH-20、半制备高效液相等色谱技术从其发酵液乙酸乙酯提取物中分离得到12个化合物。通过各种光谱分析,依次鉴定为chaetoviridins A-B(1-2),chaetoglobosins A-D(3-6),chaetoglobosin J(7),chaetoglobosin Q(8),prochaetogobosinsⅠ-Ⅲ(9-11),vibratilicin(12),其中化合物12为首次从毛壳属中分离得到。对化合物进行α-葡萄糖苷酶抑制活性测定发现,化合物12显示弱的抑制活性,其IC₅₀为(1 182.75±19.14)μg/mL。

Secondary metabolites and α-glucosidase inhibitory activities of Chaetomium globosum H6

By using α-glucosidase enzyme model in vitro, the inhibitory activity of ethyl acetate extract from mycelium and culture of Chaetomium globosum H6 on α-glucosidase enzyme was evaluated. The results showed that ethyl acetate extract of the culture had strong inhibitory activity with IC₅₀ value of (510.76±23.46)μg/mL. Twelve compounds were isolated by chromatographic technologies such as silica gel, Sephadex LH-20 and preparative HPLC. Chemical structures of compounds were identified as chaetoviridins A-B (1-2), chaetoglobosins A-D (3-6), chaetoglobosin J (7), chaetoglobosin Q (8), prochaetogobosins I-III (9-11), vibratilicin (12) by analysis of the spectroscopic data. Among them, compound 12 was isolated from Chaetomium for the first time. The inhibitory activity of α-glucosidase enzyme of the above-mentioned compounds was evaluated. Compound 12 displayed weak activity with IC₅₀ value at (1 182.75±19.14)μg/mL.