不同产地大蝉草的ITS鉴定及氨基酸和核苷成分比较

采用ITS方法对来自安徽省六安市霍山县、浙江省杭州市西湖区天竺山、浙江省台州市临海市、江苏省溧阳市平桥镇平桥山、江苏省句容市磨盘山和福建省三明市三元区中村乡6个地区的疑似蝉花样品进行分子鉴定,结果均为大蝉草Cordyceps cicadae,即蝉花。采用氨基酸自动分析仪测定其含量,由高到低依次是：浙江杭州市天竺山、浙江台州市临海、江苏溧阳市平桥山、江苏句容市磨盘山、安徽六安市霍山县和福建三明市三元。使用高效液相色谱对6个产地的蝉花的核苷进行测定,结果表明江苏平桥蝉花的N-6-(2-羟乙基)腺苷含量最高,杭州天竺山蝉花黄嘌呤和胞嘧啶含量最低。

Analyses on ITS sequence,amino acid,and nucleoside component of wild Cordyceps cicadae of different origins

ITS sequence analysis was used to determine wild Cordyceps cicadae collected from six locations in China, including Tianzhu Mountain in Hangzhou, Zhejiang Province, Huoshan County in Liu’an, Anhui Province, Mopan Mountain in Jurong City, Jiangsu Province, Linhai City in Zhejiang Province, Pingqiao Mountain in Liyang City, Jiangsu Province, and Zhongcun Village in Sanming City, Fujian Province. All these wild samples were identified to be C. cicadae. Amino acid analysis of these wild C. cicadae indicated that amino acid content was the highest in the samples from Tianzhu Mountain, followed by the sample from Linhai city, Pingqiao Mountain, Mopan Mountain, Huoshan County and Zhongcun Village. Nucleoside analysis showed that N6-(2-hydroxyethyl)-adenosine content was the lowest in the samples from Pingqiao Mountain. Both xanthine and cytosine content were the lowest in the samples from Tianzhu Mountain.