| 耐冷皮壳正青霉一种木聚糖酶的纯化与性质研究 |
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| 引用本文： | 朱会芳,周鹏,闫巧娟,江正强.耐冷皮壳正青霉一种木聚糖酶的纯化与性质研究[J].菌物学报,2010,29(4):536-541. |
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| 作者姓名： | 朱会芳 周鹏 闫巧娟 江正强 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学工学院,北京,100083 |
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| 基金项目： | 高等学校博士学科点专项科研基金资助课题；全国优秀博士学位论文作者专项资金（No. 200555） |
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| 摘 要： | 研究了耐冷皮壳正青霉Eupenicillium crustaceum一种木聚糖酶的纯化和酶学性质。采用硫酸铵沉淀和阴离子交换层析的方法,从耐冷皮壳正青霉液体发酵液中分离纯化出一种亚基分子量35kDa的木聚糖酶。酶学性质研究表明,酶的最适pH值为5.5,在pH4.5-6.5范围内具有较高的催化活性。最适温度为50℃,20℃下酶活为最高酶活的40%。Ag+和Fe2+大幅度提高木聚糖酶的酶活,而Mn2+和Hg2+强烈抑制木聚糖酶的活性。同时,该木聚糖酶具有严格的底物特异性。
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| 关 键 词： | 木聚糖酶 低温 真菌 酶学性质 |
Purification and characterization of a xylanase from psychrotrophic Eupenicillium crustaceum |
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| Authors: | ZHU Hui-Fang ZHOU Peng YAN Qiao-Juan and JIANG Zheng-Qiang |
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| Institution: | College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;College of Engineering, China Agricultural University, Beijing 100083, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | A xylanase from psychrotrophic Eupenicillium crustaceum was purified and characterized.The xylanase was purified to homogeneity by ammonium precipitation and anion-exchange chromatography and its subunit molecular mass was estimated to be 35kDa by SDS-PAGE.The xylanase showed apparent optimal activity at pH 5.5 and 50℃.It retained more than 80% of its maximum activity at pH 4.5-6.5 and retainded 40% of its maximum activity at 20℃.The enzyme was greatly activated by Ag+ and Fe2+,and strongly inhibited by Mn2+ and Hg2+.The xylanase displayed a strict substrate specificity. |
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| Keywords: | xylanase low temperature fungi enzymatic characterization |
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