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甘肃天水地区小麦条锈菌自然群体DNA指纹分析
引用本文:郑文明,陈受宜,康振生,王阳,吴立人,李振岐.甘肃天水地区小麦条锈菌自然群体DNA指纹分析[J].菌物学报,2005,24(2):199-206.
作者姓名:郑文明  陈受宜  康振生  王阳  吴立人  李振岐
作者单位:1. 西北农林科技大学植保学院,陕西杨凌,712100;河南农业大学植保学院,郑州,450002
2. 中科院遗传发育研究所,北京,100101
3. 西北农林科技大学植保学院,陕西杨凌,712100
4. 中国农科院植保所,北京,100094
基金项目:国家重点基础研究发展规划项目(G200016201) 国家杰出青年基金项目(30125031)
摘    要:对1997—1999连续三年采自甘肃省天水地区16个地点的244个小麦条锈菌分离系进行了PSR331S3/Bg1Ⅱ指纹分析,共鉴定出表现型185个,遗传多样性Shannon指数M=4.467, 加权修正值M*=0.9106,显现出高度的遗传多样性水平;其中三个主要采样点凤凰、甘谷和麦积山病菌群体M值分别为3.5720、3.0268和3.4186,加权修正值M*分别为0.9330、0.8513和0.8610。三个群体之间的遗传分化系数GST=0.03167,显示出较低的遗传分化水平。本文以多拷贝的RFLP标记为手段,揭示了小麦条锈菌作为一种活体寄生的远程气传病原真菌,其越夏栖息地关键区(天水地区)自然群体存在丰富的遗传多样性,不同海拔生境对病菌群体结构有显著的影响。但是,地区内亚群体间分化水平很低,表明区域内菌源交流十分频繁。

关 键 词:越夏栖息地  遗传多样性  遗传分化  表现型  菌源交流
文章编号:1672-6472(2005)02-0199-0206
修稿时间:2004年4月20日

DNA FINGERPRINTING OF NATURAL POPULATION OF PUCCINIA STRIIFORMIS F. SP. TRITICI IN TIANSHUI AREA
Authors:ZHENG Wen-Ming  CHEN Shou-Yi  KANG Zhen-sheng  Wang Yang  WU Li-ren  LI Zhen-Qi
Abstract:By employing PSR331S3 as a probe and BglⅡ as restriction endonuclease, the fingerprints of Puccinia striiformis f. sp. tritici population containing 244 isolates from Tianshui area of Gansu province were investigated. Totally 185 phenotypes of fingerprint were identified in the tested pathogen collection, the genetic polymorphism Shannon M value was 4.467, and normalized M* was 0.9106. The population M values of isolates collected from Fenghuang, Gangu and Maiji mountain within Tianshui area were 3.5720, 3.0268 and 3.4186, while their normalized M* were 0.9330, 0.8513 and 0.8610 respectively. This research revealed that the population of P. striiformis f. sp. tritici in Tianshui area was greatly rich in genetic variations. The elevation may cause the alterations of pathogen population diversity. The genetic differentiation coefficient GST value among three main locations within Tianshui area was 0.03167, which indicated that there existed a very low differentiation level among them, and also meant the exchange for the pathogen within the area was undergone very often.
Keywords:Over-summer habitat  genetic diversity  genetic differentiation  phenotype  pathogen exchange
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