| 食用菌菌丝体中糖醇类成分含量测定的研究 |
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| 引用本文： | 周帅,薛俊杰,贾薇,张劲松,杨焱,唐庆九.食用菌菌丝体中糖醇类成分含量测定的研究[J].菌物学报,2013,32(5):862-867. |
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| 作者姓名： | 周帅 薛俊杰 贾薇 张劲松 杨焱 唐庆九 |
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| 作者单位： | 农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403;农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403;农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403;农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403;农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403;农业部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字（2009）第2-2号] |
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| 摘 要： | 采用高效阴离子色谱-脉冲安培法(HAPEC-PAD)对食用菌菌丝体中糖醇类成分赤藓糖醇、阿糖醇和甘露醇进行分析,并对赤藓糖醇测定的方法进行考察,结果表明该方法对赤藓糖醇的分析具有灵敏度高、分离度好、检测效率高等特点。不同食用菌菌丝体均含有糖醇类成分,但含量和种类差异大,同时发现百令胶囊的测定结果与药典有较大差异,主要含有赤藓糖醇,而非甘露醇。此研究为糖醇类成分的质量标准的建立奠定了基础。
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| 关 键 词： | 百令胶囊 赤藓糖醇 甘露醇 阿糖醇 高效阴离子色谱-脉冲安培法 食用菌 |
Alditol content in edible fungal mycelia |
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| Authors: | ZHOU Shuai XUE Jun-Jie JIA Wei ZHANG Jing-Song YANG Yan and TANG Qing-Jiu |
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| Institution: | National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China |
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| Abstract: | The contents of erythritol, mannitol and arabitol in edible fungal mycelia are analyzed by the method of high performance anion chromatography-pulsed amperometric detection (HAPEC-PAD). Methodology of detecting erythritol is specially investigated and it is found that this method shows high sensitivity, separability and efficiency. The contents of alditols in mycelium of different edible fungal species were found to be variable. Using this method, corbrin capsule is also tested and the results show that the main alditol contained is erythritol but not mannitol. These studies could be regarded as the foundation for quality standardization of alditols in fungal mycelia. |
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| Keywords: | corbrin capsule erythritol mannitol arabitol high performance anion chromatography-pulsed amperometric detection edible fungi |
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