广东湛江湾可培养丝状真菌分布状况的初步调查

通过一次航行对广东湛江湾19个站位的表层和底层海水进行采样。经过对样品的分离纯化,共获得253株丝状真菌菌株。通过测序获得了121个rDNA-ITS序列,并已提交给GenBank。经统计分析发现,湛江湾表层和底层海水真菌数量总体上表现为由湾内向湾口逐渐减少的水平分布格局;湛江湾表层丝状真菌的数量略多于底层,在海水表层和底层中真菌种类和数量的分布规律相似。根据形态特征及rDNA-ITS的Blast同源性分析,这些菌株分属于18个属和32个分类单元,其中包括7个海洋真菌新记录属。结果表明,湛江湾丝状真菌多样性Shannon指数达2.75,物种优势度变化范围为30.90%–0.02%。枝孢属Cladosporium的优势度最高,为湛江湾优势种群,其次是青霉属Penicillium、侧齿霉属Engyodontium、曲霉属Aspergillus、枝顶孢属Acremonium等。湛江湾表层和底层海水真菌群落的Jaccard相似系数为0.42。

A preliminary investigation on distribution of cultivable filamentous fungi in Zhanjiang Bay, Guangdong Province

Totally 253 fungal isolates were cultured from surface and bottom seawater samples collected at 19 sites in the Zhanjiang Bay, Guangdong Province. After rDNA sequencing, 121 rDNA-ITS sequences were obtained, they were all handed to and accepted by GenBank. Fungal species diversity and magnitude in sea surface layers and bottom layers showed gradual decrease from inner region of the bay to the open sea. These fungal isolates were identified as 32 taxa belonging to 18 genera including seven newly recorded genera for Chinese marine environment. The results indicated that the filamentous fungi in Zhanjiang Bay were diverse with Shannon index 2.75 and 30.90% to 0.02% of species dominance indices. The genus Cladosporium was dominant, followed by Penicillium, Engyodontium, Aspergillus, and Acremonium. The Jaccard index of these fungi in the surface and bottom layers was 0.42.