白黄侧耳Pleurotus cornucopiae微卫星间区(ISSR)分析

本试验对我国1982年至2004年22年间栽培的白黄侧耳Pleurotus cornucopiae 30个菌株进行了锚定ISSR分析,试验表明,引物P4和P5都能对白黄侧耳P.cornucopiae进行多态性扩增,P4将供试菌株扩增出45个条带,大小在200～20000bp,P5将供试菌株扩增出39个条带,大小在500～15000bp,扩增出的条带100%具多态性。聚类分析在遗传相似性61%的水平下将30个供试菌株划分为15个类群,即15个具一定遗传差异的菌株;具有相同ISSR图谱、遗传相似性程度100%的可能为同一菌株,属于同物异名。试验表明我国的食用蕈菌野生环境面临人工栽培种质的污染,采自河北、山东、云南自然环境下的白黄侧耳P.cornucopiae与此前大量栽培的一些商业品种具完全相同的ISSR指纹图谱,聚类分析相似性系数100%。

Inter simple sequence repeat analysis for Pleurotus cornucopiae

The anchored ISSR analysis of Pleurotus cornucopiae was carried out for identification of the thirty cultures cultivated from 1982 to 2004 in China. The polymorphic DNA fragments could be produced by both of the primer P4 and P5 for the tested cultures of P. cornucopiae. Forty-five bands arose from primer P4, 200~20000 bp in length and thirty-nine bands arose from primer P5, 500~15000 bp in length. All of the bands brought polymorphic among the tested strains. The thirty cultures of P. cornucopiae were divided into 15 groups, i.e. 15 stains under similarity of 61% by cluster analysis. The cultures with the same ISSR profiles and 100% similarity of cluster analysis probably belong to the same strain. The results show that the environment of the wild mushrooms is faced with the contamination of germplasms from the commercial varieties. The ISSR profiles of wild P. cornucopiae in Hebei, Shandong and Yunnan are equal to those of commercial varieties cultivated before, and the similarity analyzed by UPGAMA is 100%.