葡萄座腔菌属ITS-nrDNA的分子系统学分析

对收集的22株和GenBank下载的40株葡萄座腔菌Botryosphaeria ITS序列构建了MP(Maximum parsimony)进化树。在以Leucostoma为外群的情况下,来自我国(主要是陕西)的葡萄座腔菌树木溃疡病菌总体上可分为2个大群,BDGroup1与GenBank上报道的B.dothidea被聚在一起,BD Group2菌株全部来自我国关中咸阳,独立成群,与Botryosphaeria属其他种亲缘关系远。培养特征、分生孢子大小和致病性测定显示:来自我国的苹果轮纹病菌、苹果干腐病菌、梨轮纹病菌、桃树流胶病菌同B.dothidea亲缘关系近,在MP树中聚在一起(BD Group1)。ITS序列分析结果支持B.dothidea与B.berengeriana为同物异名的观点。B.ribis与B.parva亲缘关系近,而与B.dothidea亲缘关系远,支持B.ribis与B.dothidea为不同种的观点。

Phylogeny of Botryosphaeria species based on ITS-nrDNA sequences

Based on maximum parsimony phylogeny tree of ITS-nrDNA sequences of 22 isolates collected from Shaanxi Province and 40 isolates downloaded from GenBank, Botryosphaeria species from China were clustered into two groups: BDGroup1 was composed of B. dothidea isolates, and BDGroup2 from Guanzhong region of Shaanxi Province was a distant related group. Cultivation characteristics, conidial size and pathogenicity of the Chinese isolates indicated that pathogens of apple ring rot disease, apple stem dry rot disease, pear ring rot disease and peach gummosis had high homogenous with B. dothidea, and they were all clustered in BDGroup1, supporting the idea that B. dothide and B. berengeriana are synonymous. The MP (Maximum parsimony) tree also showed that B. ribis was not the synonym species of B. dothidea, and B. ribis was more homogenous with B. parva Pennycook & Samuels. The other Botryosphaeria species were also discussed in this paper.