尖顶羊肚菌胞外多糖提取物对皮肤成纤维细胞增殖和衰老的影响

不同浓度尖顶羊肚菌胞外多糖提取物作用人皮肤成纤维细胞（human skin fibroblasts，HSF），检测对HSF细胞形态、细胞增殖、衰老相关β-半乳糖苷酶活性、羟脯氨酸含量的影响，探究尖顶羊肚菌胞外多糖提取物对HSF增殖和衰老的影响。结果显示，125μg/mL尖顶羊肚菌胞外多糖提取物使HSF细胞活力增加了25.2%，羟脯氨酸含量增加了12.1%，β-半乳糖苷酶活性降低了48.1%。说明适宜浓度尖顶羊肚菌胞外多糖提取物具有促进HSF细胞增殖、胶原蛋白合成，延缓细胞衰老的作用。

Effects of exopolysaccharide extract from Morchella conica on proliferation and aging of human skin fibroblasts

The effects of exopolysaccharide extract from Morchella conica (EEMC) on cell senescence were examined in human skin fibroblasts (HSF). Cell morphology, proliferation, senescence-associated β-alactosidase (SA-β-Gal) activity, and hydroxyproline content of cells treated by exopolysaccharide were measured. HSF cell viability and hydroxyproline content increased by 25.2% and 12.1% respectively, and β-galactosidase activity decreased by 48.1%, under the treatment of EEMC at the concentration of 125μg/mL. It was indicated that EEMC promoted the proliferation of HSF and the production of collagen, and thus deferred the cell aging.