| Ganoderma lucidum U-281漆酶催化偶氮染料活性黑5脱色 |
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| 引用本文： | 赵世光,张焱,薛正莲,王洲,汤明礼.Ganoderma lucidum U-281漆酶催化偶氮染料活性黑5脱色[J].菌物学报,2012,31(6):867-877. |
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| 作者姓名： | 赵世光 张焱 薛正莲 王洲 汤明礼 |
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| 作者单位： | 1. 安徽工程大学生物与化学工程学院 安徽芜湖241000;微生物发酵安徽省工程技术研究中心 安徽芜湖241000
2. 中国科学院离子束生物工程学重点实验室 安徽合肥230031 |
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| 基金项目： | 安徽省高校省级自然科学研究项目（No. KJ2011B025）；安徽工程大学引进人才科研启动基金（No. 2007YQQ007） |
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| 摘 要： | 漆酶在纺织染料脱色及印染废水处理领域有着广阔的应用前景。活性黑5是纺织印染中应用广泛的偶氮类活性染料,结构复杂,生物降解性低。以灵芝菌Ganoderma lucidum U-281所产漆酶对活性黑5进行氧化脱色,采用单因素逐一优化方法得到了U-281漆酶催化活性黑5脱色的工艺参数:染料初始浓度25mg/L、漆酶用量2.0U/mL、铜离子添加量40mmol/L、pH6.0、40℃。在优化条件下,4h可使RB5脱色62.34%,24h可完成90%以上的脱色效果。
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| 关 键 词： | 白腐真菌 合成染料 酶促氧化 脱色降解 |
Decolorization of reactive black 5 catalyzed by laccase from Ganoderma lucidum U-281 |
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| Authors: | ZHAO Shi-Guang ZHANG Yan XUE Zheng-Lian WANG Zhou and TANG Ming-Li |
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| Institution: | College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China
Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Wuhu, Anhui 241000, China;College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China
Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Wuhu, Anhui 241000, China;College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China
Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Wuhu, Anhui 241000, China;College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China
Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Wuhu, Anhui 241000, China;Key Laboratory of Ion Beam Bio-engineering, Chinese Academy of Sciences, Hefei, Anhui 230031, China |
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| Abstract: | Laccase has an extensive application on the decoloration of textile dyes and waste-dyeing-water treatment. Reactive black 5 (RB5), a representative azo dye, has complex structure and is low-biodegradable. In this paper, laccase produced by Ganoderma lucidum U-281 was used to decolorize RB5. The optimal parameters for RB5 decolorizing catalyzed by laccase from U-281 were seriatim obtained by single factor optimization method. The results were showed as follows: 25mg/L of initial dye concentration, 2.0U/mL of laccase dosage, 40mmol/L of Cu2+ addition and pH 6.0 at 40℃. Under the optimized conditions, 62.34% and more than 90% of RB5 was decolorized in 4 hours and 24 hours respectively. |
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| Keywords: | white rot fungi synthetic dyes enzymatic oxidation decoloration and degradation |
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